| 曲海源,徐缓,韩洪波,陈骐,刘静,邓意辉,徐克.αvβ3整合素受体靶向性超顺磁性脂质体的建立及体外磁共振观察[J].中国医学影像技术,2009,25(6):969~972 |
| αvβ3整合素受体靶向性超顺磁性脂质体的建立及体外磁共振观察 |
| Development of αvβ3 integrin targeted superparamagnetic liposome and in vitro MR observation |
| 投稿时间：2008-12-05 修订日期：2009-02-16 |
| DOI： |
| 中文关键词: 超顺磁性脂质体 精氨酸-甘氨酸-天冬氨酸 整合素αvβ3 血管生成 磁共振成像 |
| 英文关键词:Superparamagnetic liposome Arginyl-glycyl-aspartic acid Integrin alphaVbeta3 Angiogenesis Magnetic resonance imaging |
| 基金项目:国家自然科学基金(30500139)。 |
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| 中文摘要: |
| 目的 合成以RGD短肽为亲和成分的靶向性超顺磁性长循环脂质体,并通过体外细胞学实验确定其受体靶向性,探讨其用于肿瘤受体成像的可能性。方法 采用薄膜法分别合成表面连接和不连接RGD的靶向性和非靶向性长循环脂质体;以流式细胞分析及共聚焦激光扫描显微镜观察靶向对比剂对HUVEC细胞的特异性标记。结果 ①合成的超顺磁性脂质体粒径在150 nm左右,具有较高的弛豫率;②靶向性对比剂对αvβ3整合素受体具有较高的特异性亲和力;可通过受体介导的内吞作用进入细胞内;③靶向对比剂与细胞结合后,可以明显降低细胞的信号。结论 RGD—超顺磁性长循环脂质体具有较高的弛豫率,能与αvβ3整合素受体特异性结合,在靶点特异性浓聚,并能经MR扫描证实。 |
| 英文摘要: |
| Objective To synthesize the targeted superparamagnetic liposome with RGD oligopeptide as main binding components, and to assess its targeted ability and possibility of its application in receptor imaging with in vitro fluorescence microscopy. Methods SPIO liposomes conjugated with or without Arg-Gly-Asp (RGD) peptides were made by film method. The binding and uptake of RGD-liposomes by HUVECs were analyzed with flow cytometry and confocal laser scanning microscopy (CLSM). Results ①The superparamagnetic liposome had high relaxation rate with the size of about 150 nm; ②Targeted contrast medium had specific affinity for αvβ3 integrin and could enter cells with receptor-mediated endocytosis; ③Cell signal reduced significantly when targeted contrast medium combined with cells. Conclusion The RGD targeted super-paramagnetic liposome has high relaxation rate. It can bind particularly with αvβ3 integrins and be detected with in vitro MR imaging. |
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