| 白园园,修建成,宾建平,张川,查道刚,刘伊丽.超声靶向破坏微泡介导骨髓间充质干细胞移植促进大鼠血管新生[J].中国医学影像技术,2009,25(6):942~944 |
| 超声靶向破坏微泡介导骨髓间充质干细胞移植促进大鼠血管新生 |
| Targeted microbubble destruction with ultrasound augments neovascularization by bone marrow mesenchymal stem cells transplantation |
| 投稿时间：2008-09-22 修订日期：2009-02-20 |
| DOI： |
| 中文关键词: 超声检查 微泡 骨髓间充质干细胞 血管新生 鼠 |
| 英文关键词:Ultrasonography Microbubble Bone marrow mesenchymal stem cells Neovascularization Mice |
| 基金项目:国家自然科学基金(30400419)。 |
|
| 摘要点击次数: 2273 |
| 全文下载次数: 800 |
| 中文摘要: |
| 目的 探讨超声破坏微泡介导骨髓间充质干细胞(MSCs)移植在大鼠缺血骨骼肌中的作用。 方法 24只Wister大鼠离断右侧股动脉7 d后分为4组:①超声+微泡组(US+MB);②干细胞静脉移植组(MSCs-iv);③超声+微泡＋干细胞静脉移植组(US+MB+MSCs-iv);④对照组。处理后第7 d取局部缺血骨骼肌行免疫组化检测。 结果 US+MB+MSCs-iv组缺血骨骼肌可见较多移植的MSCs,其新生血管数量较其他组明显增加。 结论 超声靶向破坏微泡有可能成为一种增强MSCs移植和促进血管新生的新方法。 |
| 英文摘要: |
| Objective To examine the effects of targeted microbubble destruction with ultrasound combined with bone marrow mesenchymal stem cells (MSCs) transplantation into ischaemic tissues of rat hind limb ischaemia. Methods Unilateral hind limb ischaemia was surgically induced in 24 Wister rats. Seven days postoperatively, rats were divided into 4 groups: ultrasound+microbubble group (US+MB), MSCs-iv group (MSCs-iv), ultrasound+microbubble+MSCs-iv group (US+MB+MSCs-iv) and control group (each n=6). Muscles were obtained 7 d after experiment and were detected by immunohistochemistry. Results In US+MB+MSCs-iv group, numbers of MSCs and neovascularization by immunohistochemistry were greater in the targeted muscles than that in other groups. Conclusion US-targeted microbubble destruction may be a useful technique to enhance MSCs transplantation and neovascularization. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
