| 伍星,王志刚,唐毅,李攀,朱叶锋,冉海涛,刘学兵.携VEGFR2单抗靶向微泡评价小鼠肿瘤新生血管[J].中国医学影像技术,2009,25(6):932~934 |
| 携VEGFR2单抗靶向微泡评价小鼠肿瘤新生血管 |
| Assessment of tumor angiogenesis using contrast enhanced ultrasound with VEGFR2-targeted microbubbles |
| 投稿时间：2008-11-28 修订日期：2008-12-21 |
| DOI： |
| 中文关键词: 超声检查,介入性 肿瘤 血管新生 血管内皮生长因子受体2 |
| 英文关键词:Ultrasonography, interventional Neoplasms Angiogenesis Vascular endothelial growth factor receptor-2 |
| 基金项目:国家自然科学基金重点项目(30430230),国家高技术研究发展计划(863计划,2006AA02Z4F0),国家自然科学基金青年基金(60502002)。 |
|
| 摘要点击次数: 2505 |
| 全文下载次数: 759 |
| 中文摘要: |
| 目的 探讨携带抗血管内皮生长因子受体2(VEGFR2)单克隆抗体的超声微泡造影剂(MBv)评价小鼠肿瘤新生血管的可行性。方法 以生物素-亲和素桥接法构建MBv。免疫荧光法体外鉴定其性质。建立小鼠H22肿瘤模型,分别注射MBv和普通脂质微泡(MBc)进行超声造影检查。定量分析造影图像视频强度变化,描绘时间-强度曲线,并行肿瘤组织VEGFR2免疫组化检测。结果 成功构建偶联抗小鼠VEGFR2单抗的MBv。造影结果表明MBv组较MBc组达峰时间提前,峰值较高,持续时间更长。免疫组化证明瘤体内新生血管内壁VEGFR2高度表达。结论 应用MBv结合超声造影可较好地评估肿瘤新生血管状况。MBv是良好的肿瘤靶向造影剂。 |
| 英文摘要: |
| Objective To investigate the feasibility of contrast enhanced ultrasound (CEU) with VEGFR2-targeted microbubbles (MBv) to assessment tumor angiogenesis. Methods MBv was prepared by biotin-avidin conjugation method. The conjugation was demonstrated by immunofluorescence. H22 tumor models in mice were established and underwent CEU examination with MBv or control microbubbles (MBc). Sonograms were analyzed by calculation of the normalized video intensity (VI), and time-intensity curve (TIC) was depicted according to VI changes. Tumor samples were harvested for analysis of VEGFR2 expressions by immunohistochemistry. Results MBv was demonstrated bounding with anti-VEGFR2 antibody effectively. TIC showed that the time to maximum echogenicity was earlier, peak echogenicity was higher, and the time to wash out was longer in MBv contrast group compared with that in MBc group. Immunohistochemistry showed VEGFR2 was highly expressed in novel vessel walls. Conclusion Tumor angiogenesis can be well evaluated using CEU with MBv. MBv can be regarded as a potential tumor targeted contrast agent. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
