| 李宗芳,刘流,田伟,滕皋军,张宇,葛玉卿,王福科.MRI观察超顺磁性纳米颗粒标记的AnnexinV体外检测细胞凋亡[J].中国医学影像技术,2009,25(5):734~737 |
| MRI观察超顺磁性纳米颗粒标记的AnnexinV体外检测细胞凋亡 |
| MRI observation of apoptotic cells using superparamagnetic iron oxide nanoparticles labeled Annexin V in vitro |
| 投稿时间：2008-11-26 修订日期：2009-01-08 |
| DOI： |
| 中文关键词: 磁共振成像 凋亡 膜联蛋白 V |
| 英文关键词:Magnetic resonance imaging Apoptosis Annexin V |
| 基金项目: |
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| 中文摘要: |
| 目的 利用MRI观察超顺磁性纳米颗粒(SPIO)标记的膜联蛋白 V(Annexin V)体外检测凋亡细胞的能力。方法 对5管不同的细胞进行MRI和普鲁士蓝染色,分别为:A管:未经H2O2诱导凋亡处理的细胞与SPIO孵育;B管:经H2O2诱导凋亡处理的细胞与SPIO孵育;C管:经H2O2诱导凋亡处理的细胞的空白管;D管:经H2O2诱导凋亡处理的细胞与Annexin V-SPIO孵育;E管:未经H2O2诱导凋亡处理的细胞与Annexin V-SPIO孵育。结果 5管间总体比较MR信号值差异有统计学意义(F=569.38,P＜0.01),其中A、B、C三管的MR信号值差异无统计学意义,D和B管、E和A管、D和E管的MR信号值比较差异均有统计学意义。普鲁士蓝染色显示Annexin V-SPIO可与凋亡细胞膜特异性结合。结论 Annexin V-SPIO可与体外凋亡细胞特异性结合,并引起磁共振信号改变。 |
| 英文摘要: |
| Objective To evaluate the feasibility of imaging apoptotic cells in vitro by MRI with SPIO labeled Annexin V. Methods Five tubes (A-E) containing different cells were scanned with MR and then stained with Prussian blue, including tube A (cells not treated with H2O2 before incubated with SPIO), tube B (cells treated with H2O2 before incubated with SPIO), tube C (cells not treated with H2O2 and as control tube), tube D (cells treated with H2O2 before incubated with Annexin V-SPIO) and tube E (cells not treated with H2O2 before incubated with Annexin V-SPIO). Results There were statistically significant differences among five tubes on the signal intensity of MR (F=569.38, P＜0.01), especially between tube D and tube B, tube E and tube A, tube D and tube E. Prussian blue stain revealed SPIO labeled Annexin V could bind specifically to apoptotic cells. Conclusion SPIO labeled Annexin V can bind specifically to apoptotic cells, inducing the signal intensity changes of MRI. |
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