| 张秀明,戴峰,姚群立,徐清宇,滕皋军.大鼠急性脑缺血再灌注模型的7.0T MR成像研究[J].中国医学影像技术,2009,25(5):731~733 |
| 大鼠急性脑缺血再灌注模型的7.0T MR成像研究 |
| Experimental model of acute cerebral ischemia and reperfusion assessed by 7.0T magnetic resonance imaging |
| 投稿时间：2008-12-18 修订日期：2009-01-21 |
| DOI： |
| 中文关键词: 磁共振成像 缺血再灌注 磁共振血管成像 |
| 英文关键词:Magnetic resonance imaging Ischemia and reperfusion Magnetic resonance angiography |
| 基金项目: |
|
| 摘要点击次数: 1664 |
| 全文下载次数: 845 |
| 中文摘要: |
| 目的 大鼠急性脑梗死缺血再灌注模型的制作和不同时间段7.0T MR成像特点研究。 方法 将45只SD大鼠随机分成两组,假手术组大鼠5只,模型组40只,其中模型组随机平均分成5组。于术后2、4、6、12、24 h行MR扫描,对磁共振影像和病理结果进行分析。 结果 以神经症状及磁共振血管成像(MRA)判断造模成功的36只大鼠,于术后2 h弥散加权像(DWI)见高信号改变,同时T2弛豫率升高,并随时间延长不断扩大及增高,6 h后T2加权像(T2WI)出现高信号,于12 h内T2WI高信号区面积小于DWI高信号区面积(P＜0.05)。T1WI表现为相应区域脑组织肿胀,TTC染色证实为梗死区,病理观察见该供血区细胞溶解。头颅血管成像(MRA)示阻断侧大脑中动脉信号消失,拔线后血流信号恢复。模型成功率为90%。 结论 线栓法制作大鼠急性脑梗死及再灌注模型稳定可行。7.0T MRI反映脑组织改变具有高度的时间、空间敏感性及特异性,对脑梗死诊断具有一定意义。 |
| 英文摘要: |
| Objective To establish an experimental model of acute cerebral ischemia and reperfusion by occluding middle cerebral artery in rat, and to observe the imaging charicteristics of this model with PharmaScan 7.0T magnetic resonance system. Methods Forty-five rats were divided into two groups randomly. Five for sham operation group, forty were divided into five groups randomly and examined by MR on 2, 4, 6, 12, 24 hours after operation and observed pathologically. Results Thirty-six rats were defined successful model with neurological scores and magnetic resonance angiography (MRA), in which high signal on diffusion weighted imaging (DWI) were detected on 2 h, and T2 relaxtime increased at the same time. The range of high signal and the value of T2 relaxtime extended with the passage of time. High signal on T2WI was found on 6 h, but the range of high signal in T2WI was less than that in DWI before 12 h (P＜0.05). Swelling of brain tissue was depicted on T1WI, which were proved infarcted area by TTC, as well as infracted or ischemic changes were observed in the region supplied by the occluded artery. MRA showed the signal of the occluded artery disappeared and resumed after pulled out the line embolism. The successful rate of making model was 90%. Conclusion Modified method of constructing model is reliable and stable. 7.0T MR has high time and spatial sensitivity and specificity, and has some significance in diagnosis of cerebral ischemia and reperfusion. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
