| 周碧婧,何慧瑾,冯晓源,张善中,杨艳梅.大鼠胰岛细胞的体外和体内MR成像方法[J].中国医学影像技术,2009,25(1):40~42 |
| 大鼠胰岛细胞的体外和体内MR成像方法 |
| In vitro and in vivo MR imaging of rat pancreatic islet cells |
| 投稿时间：2008-07-22 修订日期：2008-09-29 |
| DOI： |
| 中文关键词: 胰岛 细胞移植 磁共振成像 |
| 英文关键词:Islets of Langerhans Cell transplantation Magnetic resonance imaging |
| 基金项目: |
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| 摘要点击次数: 2688 |
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| 中文摘要: |
| 目的 探讨MRI对SPIO标记的大鼠胰岛细胞在体外及体内的扫描参数及序列的选用。方法 联合应用SPIO和多聚左旋赖氨酸(PLL),通过受体介导的内吞作用标记胰岛细胞。采用GE 3.0T Signa Excite磁共振扫描仪,配合3英寸小动物线圈作GRE序列T2*W成像,比较SPIO标记的胰岛细胞与未标记的细胞,再分别采用两种具有不同分辨率的扫描方案进行扫描,比较图像的差异。而后将两种细胞分别移植入大鼠体内,比较在体内的差异。对标记后的大鼠分别用FSE T2W序列和GRE T2*W序列扫描,比较各序列的敏感性。 结果 无论是体外还是体内,只有SPIO标记后的胰岛细胞可以被MRI监测到,表现为较明显的低信号点。采用更高分辨率的参数扫描后所得到的胰岛图像更为清晰。GRE T2*WI较FSE T2WI序列对SPIO的监测更敏感。结论 MRI能对SPIO标记的胰岛细胞进行成像,可用于活体,实时监测胰岛细胞移植术后移植物的存活及排异情况。 |
| 英文摘要: |
| Objective To track SPIO-labeled cells using MRI in vitro and in vivo, in order to find appropriate parameters and sequence. Methods Pancreatic islet cells of rat were labeled with SPIO combined with PLL by means of receptor-mediated endocytosis, and were scanned on a GE 3.0T Signa Excite MR scanner equipped with an animal coil for a standard T2*-weighted gradient echo sequence. The images of SPIO-labeled cells were compared with that of the unlabeled. Two scan protocols with different resolutions were used to scan the labeled cells. SPIO-labeled and unlabeled cells were transplanted into the rats’ liver respectively, and their MR images were compared. FSE T2WI sequence and GRE T2*WI sequence were used to scan the SPIO-labeled cells, and the sensitivity of images was compared. Results Only SPIO-labeled pancreatic islet cells were seen on MRI as distinct hypointense spots both in vitro and in vivo. The SPIO-labeled pancreatic islet cells could be more clearly seen on higher resolution images. GRE T2*WI was more sensitive on the detection of SPIO than FSE T2WI. Conclusion MRI can be used to track SPIO-labeled islet cells both in vitro and in vivo, and provide great value to detect the survival status and rejection of grafts after transplantation. |
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