| 郑薇,谭建,李玮,李宁,许静.裸鼠模型的人钠/碘转运体基因转染人大细胞肺癌介导放射性核素显像[J].中国医学影像技术,2009,25(1):7~9 |
| 裸鼠模型的人钠/碘转运体基因转染人大细胞肺癌介导放射性核素显像 |
| Radionuclide imaging of large cell lung cancer in nude mice mode bytransfected human sodium/iodide symporter gene |
| 投稿时间：2008-10-20 修订日期：2008-12-03 |
| DOI： |
| 中文关键词: 人钠/碘转运体基因 转染 放射性核素 人大细胞肺癌 |
| 英文关键词:Human sodium/iodide symporter gene Transfect Radioactive nuclide Human large cell lung cancer |
| 基金项目:天津市应用基础及前沿技术研究计划(08JC2DJC23900)。 |
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| 中文摘要: |
| 目的 在动物体内实验观察人钠/碘转运体(hNIS)基因转染人大细胞肺癌介导放射性核素显像是否可行。 方法 ①利用重组质粒以脂质体转染法将hNIS基因转染入人大细胞肺癌H460细胞系中,获得稳定表达hNIS的细胞株(hNIS-H460)。②用hNIS-H460细胞株建立大细胞肺癌荷瘤裸鼠模型,进行放射性核素99mTcO-4显像和131I显像。 结果 ①体外实验表明hNIS-H460细胞株可以摄取碘。②裸鼠大细胞肺癌(hNIS-H460)移植瘤的99mTcO-4和131I显像结果较清晰。 结论 转染后的hNIS-H460细胞具有一定的摄碘能力。裸鼠大细胞肺癌(hNIS-H460)移植瘤可以进行99mTcO-4和131I显像;99mTcO-4显像的质量优于131I显像。 |
| 英文摘要: |
| Objective To investigate the possibility of transfecting human sodium/iodide symporter (hNIS) gene into human large cell lung cancer cell line H460 to facilitate radioactive nuclide imaging in vivo. Methods ①hNIS gene was transfectted into human large cell lung cancer cell line H460 by recombinant expression plasmids with lipofectamine 2000-plasmid complexes. Subsequently the biologic functions of stably expressing hNIS gene cell line (hNIS-460) were investigated. ② Large cell lung cancer nude mice model was established, and the radioactive isotopes 99mTcO-4 and 131I imaging were performed in nude mice. Results ①The cell line hNIS-H460 could uptake 125I. The uptake of 125I was 50.97 times higher in cells hNIS-H460 than in cells H460 (t=12.36, P<0.01). ②Radioactive isotopes 99mTcO-4 and 131I imaging in xenografts of transfected group were successfully performed using ECT imaging system. Conclusion The transfected cells hNIS-H460 can uptake iodide, and the xenografts of which can be imaged in 99mTcO-4 and 131I. The quality of 99mTcO-4 imaging is better than 131I imaging. |
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