| 张萍,高云华,刘政,刘平,谭开彬.超声联合微泡诱导血管平滑肌细胞凋亡的机制研究[J].中国医学影像技术,2008,24(11):1681~1684 |
| 超声联合微泡诱导血管平滑肌细胞凋亡的机制研究 |
| Mechanism of apoptosis of vascular smooth muscle cells induced by ultrasound irradiation combined with microbubbles |
| 投稿时间：2008-02-28 修订日期：2008-08-28 |
| DOI： |
| 中文关键词: 超声检查 微泡 肌,平滑,血管 凋亡 |
| 英文关键词:Ultrasonography Microbubbles Muscle, smooth, vascular Apoptosis |
| 基金项目:全军医药卫生科研基金(06MA185)。 |
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| 中文摘要: |
| 目的 探讨低频超声联合脂质微泡诱导血管平滑肌细胞(VSMCs)凋亡的可能机制。方法 以低频连续波超声(频率1 MHz、声强0.3 W/cm2)联合脂质微泡(1 μl/ml)辐照VSMCs 120 s。分别用流式细胞仪Annexin V/PI染色、免疫细胞化学技术、荧光探针Fura-4/Am负载后激光共聚焦法检测细胞凋亡、凋亡相关蛋白表达和细胞内游离Ca2+ 浓度的变化情况。结果 与对照组和血小板衍生生长因子-BB刺激组(PDGF)比较,两组超声辐照联合微泡 的VSMCs凋亡率显著增加、Bax蛋白表达明显上调、而Bcl-2蛋白表达则显著减少(P<0.01);而且PDGF+US+MB组的细胞凋亡率明显高于US+MB组,Bax蛋白表达上调和Bcl-2蛋白表达下调的程度较US+MB组更为显著(P<0.01)。各实验干预组VSMCs内Ca2+ 浓度均较对照组显著增高(P<0.01);PDGF+US+MB组和US+MB组细胞内Ca2+ 浓度均明显低于PDGF组(P<0.01)。结论 超声联合微泡诱导VSMCs凋亡的机制可能与细胞内游离Ca2+ 浓度增加、Bax蛋白表达增加和Bcl-2蛋白表达降低有关。 |
| 英文摘要: |
| Objective To investigate the mechanism of vascular smooth muscle cells (VSMCs) apoptosis induced by ultrasound irradiation combined with liposome microbubbles. Methods Cultured VSMCs were exposed to a low-frequency continuous wave ultrasound (1 MHz, 0.3 W/cm2, 120 s) in the presence of liposome microbubbles (1 μl/ml). Apoptosis of cells were detected by flow cytometry with double staining of fluorescein isothiocyanate (FITC)-labeled Annexin V/propidium iodide (PI), and the expression of Bax and Bcl-2 were determined by immunocytochemical stain, while intracellular free calcium concentration ( i) was detected by laser confocal microscopy with fluorescent Ca2+-sensitive probe fura-4/acetoxylmethyl ester (Fura-4/AM). Results Compared with control group and platelet derived growth factor-BB treatment group (PDGF), the percentage of apoptosis and the expression of Bax increased markedly in ultrasound combined with microbubbles group (US+MB) and ultrasound combined with microbubbles and PDGF treatment group (PDGF+US+MB, P<0.01), while the expression of Bcl-2 in the two groups decreased significantly (P<0.01). Compared with (US+MB) group, the apoptosis cells, upregulation of expression of Bax and reduction of expression of Bcl-2 in (PDGF+US+MB) group were more obvious (P<0.01). i in other tested groups increased significantly, compared with that in the control. i in (US+MB) group and (PDGF+US+MB) group were lower than that in PDGF group (P<0.01). Conclusion Low-frequency ultrasound in combination with microbubbles can induce VSMCs apoptosis, which may be related to increase of i, upregulation of expression of Bax and reduction of expression of Bcl-2. |
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