| 周宁,段云友,王勇,熊华强,巴咏梅.孕期彩色多普勒超声对仔鼠肾小管上皮细胞bcl-2远期表达的影响[J].中国医学影像技术,2008,24(9):1330~1332 |
| 孕期彩色多普勒超声对仔鼠肾小管上皮细胞bcl-2远期表达的影响 |
| The remote effect of color Doppler ultrasound on expression of bcl-2 protein in offspring's renal tubule epithelial cell in rats |
| 投稿时间：2008-04-10 修订日期：2008-06-09 |
| DOI： |
| 中文关键词: 超声检查,多普勒,彩色 肾小管 上皮细胞 bcl-2 |
| 英文关键词:Ultrasonography, Doppler, color Kidney tubules Epithelial cells bcl-2 |
| 基金项目:国家自然科学基金项目(30571966)。 |
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| 中文摘要: |
| 目的 探讨彩色多普勒超声照射对中孕期仔鼠肾小管上皮细胞bcl-2蛋白表达的影响。 方法 32只孕14天SD大鼠按照辐照时间不同随机等分为四组,Ⅰ组(对照组)、Ⅱ组(照射10 min组)、Ⅲ组(照射20 min组)、Ⅳ组(照射30 min组),仪器采用Acuson公司Sequoia-512型彩色电脑声像仪,照射条件:4V1探头,二维频率3.0 MHz,彩色频率3.0 MHz,Tis=1.8,MI=1.6。照射后仔鼠生长至90 d肾脏标本,HE染色光镜观察组织结构;免疫组化ABC法检测肾脏bcl-2蛋白的表达,采用Leica Q500MC型图像分析仪进行图像分析。 结果 Ⅰ~Ⅳ组光镜下观察肾脏组织结构均未见异常;免疫组化法检测,各组仔鼠肾小管上皮内可见均匀散在的棕黄色淡染的bcl-2蛋白阳性表达,图像分析统计结果显示:Ⅱ~Ⅳ组仔鼠肾小管上皮bcl-2蛋白阳性表达强度与Ⅰ组无显著差异(P>0.05)。 结论 彩色多普勒超声照射,对仔鼠成年后肾小管上皮细胞bcl-2蛋白表达无影响。 |
| 英文摘要: |
| Objective To study the remote effect of color Doppler ultrasound (CDUS) on the expression of bcl-2 protein in offspring's renal tubule epithelial cel1. Methods Thirty-two female Spraque-Danles rats that had been pregnant for 14 days were divided into four groups averagely and randomly, in which control group (group I) was sham-irradiation, group II—IV were irradiated by diagnostic CDUS (Sequoia-512,4V1,3.0 MHz,Tis=1.8,MI=1.6) for 10 min, 20 min and 30 min respectively. The offspring's kidneys of four groups were removed when the neonatal rats were 90 d old after irradiation, HE stain was done for microscope observing and expression of bcl-2 protein was detected with immunohistochemical method. Results There were no abnormal changes among group I—IV under microscope.And there was no significant differences in expression of bcl-2 protein among group I-IV (P>0.05). Conclusion Continuous irradiation beyond 30 minutes by diagnostic CDUS may not result in decreasing expression of bcl-2 protein in offspring's renal tubule epithelial cell. |
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