| 郑道文,杨莉,宾建平,陈楚弟,陈少敏,王月刚,吴平生.亲和素桥连构建携抗P选择素单抗靶向超声微泡及体外荧光鉴定[J].中国医学影像技术,2008,24(8):1182~1185 |
| 亲和素桥连构建携抗P选择素单抗靶向超声微泡及体外荧光鉴定 |
| Construction of targeted ultrasound microbubbles with anti P selectin monoclone antibody via streptavidin bridge and evaluation of its reliability using fluorescence in vitro |
| 投稿时间：2008-05-26 修订日期：2008-06-28 |
| DOI： |
| 中文关键词: P选择素 靶向超声微泡 荧光 |
| 英文关键词:P selectin Targeted ultrasound microbubbles Fluorescence |
| 基金项目:国家“863”计划项目（2006AA02Z478）。 |
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| 摘要点击次数: 3033 |
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| 中文摘要: |
| 目地 的体外荧光方法鉴定生物素 亲和素 生物素（BSB）桥连技术构建携带抗P 选择素单抗靶向超声微泡(MB BSBp)的可靠性。 方法 不同荧光标记物分别标记MB BSBp的不同部位，观测微泡荧光强度（0、1、2和3级），以普通脂质超声微泡（MB）作对照。 结果 加入FITC荧光亲和素孵育，生物素化脂质微泡（MB B）呈现明亮的绿色荧光（3级），而MB无荧光显示（0级）。以两个浓度梯度（1∶4和1∶16）的DTAF荧光二抗（抗抗P 选择素单抗抗体）标记MB、MB B、MB BS和MB BSBp，MB BSBp在两个浓度梯度下均发出明亮的绿色荧光（3级）；而MB B、MB BS仅在1∶4时显示微弱的绿色荧光（1级），MB在两个浓度梯度下均无荧光显示（0级）。 结论 抗P 选择素单抗通过亲和素桥连有效装配在MB B表面，体外荧光法是鉴定靶向微泡配体连接可靠性的简便方法。 |
| 英文摘要: |
| ObjectiveTo investigate the reliability of targeted ultrasound microbubbles with anti mouse P selectin monoclone antibody (MB BSBp). MethodsThe targeted microbubbles were constructed using a technology of biotin avidin biotin（BSB）antibody conjugation. Different parts of MB BSBp were marked with various fluorescent markers, and common lipid ultrasound microbubbles (MB) as control. The fluorescence intensity of microbubbles was graded from 0, 1, 2 to 3. ResultsAfter incubating with FITC avidin, the biotinylated lipid microbubbles (MB B) displayed bright green fluorescence (grade 3), but MB had no any fluorescence (grade 0). MB, MB B, streptavidin biotinylated lipid microbubbles (MB BS), and MB BSBp were marked with DTAF antibody as second antibody (antibody of anti P selectin monoclonal antibody) at two concentrations (1∶4 and 1∶16 dilution). Bright green fluorescence (grade 3) were observed in MB BSBp in both concentrations, faint green fluoresence (grade 1) in MB B and MB BS only in concentration of 1∶4 dilution, but no fluorescence was seen in MB in either concentration (grade 0). ConclusionP selectin monoclonal antibody was effectively incorporated to the MB B surface through streptavidin bridge. Fluorometric in vitro is reliable and simple method for investigating the ligand conjugation of targeted microbubble. |
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