| 项飞翔,王新房,谢明星,周翔,张艳容,张丽,黄艳.辐照时间对超声破坏微泡诱导生物效应的影响[J].中国医学影像技术,2008,24(8):1173~1176 |
| 辐照时间对超声破坏微泡诱导生物效应的影响 |
| Influence of exposure time on bioeffects induced by ultrasound-mediated microbubble destruction |
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| DOI： |
| 中文关键词: 造影剂 肝细胞 生物效应 时间 |
| 英文关键词:Contrast media Hepatocyte Bioeffect Time |
| 基金项目: |
| 作者 | 单位 | | 项飞翔 | 华中科技大学同济医学院附属协和医院超声影像科,湖北省分子影像重点实验室,湖北 武汉 430022 | | 王新房 | 华中科技大学同济医学院附属协和医院超声影像科,湖北省分子影像重点实验室,湖北 武汉 430022 | | 谢明星 | 华中科技大学同济医学院附属协和医院超声影像科,湖北省分子影像重点实验室,湖北 武汉 430022 | | 周翔 | 华中科技大学同济医学院附属协和医院超声影像科,湖北省分子影像重点实验室,湖北 武汉 430022 | | 张艳容 | 华中科技大学同济医学院附属协和医院超声影像科,湖北省分子影像重点实验室,湖北 武汉 430022 | | 张丽 | 华中科技大学同济医学院附属协和医院超声影像科,湖北省分子影像重点实验室,湖北 武汉 430022 | | 黄艳 | 华中科技大学同济医学院附属协和医院超声影像科,湖北省分子影像重点实验室,湖北 武汉 430022 |
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| 中文摘要: |
| 目的 探讨不同辐照时间对诊断超声破坏国产超声造影剂全氟显诱导HL-7702的声孔效应、细胞坏死及凋亡的影响。方法 将用于诊断的超声辐照含全氟显微泡的HL-7702细胞悬液,频率2 MHz,MI为1.9,分为对照组及实验组,对照组未经超声辐照,实验组分别设置不同的辐照时间为30 s、1、5、10和20 min。辐照后观察大分子荧光物质进入细胞情况以检测声孔效应,检测细胞活力及凋亡情况。结果 ①荧光染色结果:各实验组与对照组比较差异均具有统计学意义(P<0.05);1 min组与5 min组间及5 min组与10 min组间差异具有统计学意义(P<0.05)。而10 min组与20 min组间差异不具有统计学意义(P>0.05)。②细胞杀伤结果:与对照组比较,10 min组及20 min组细胞死亡比率差异均具统计学意义(P<0.05)。相邻组间比较,5 min组与10 min及10 min组与20 min组间差异具统计学意义(P<0.05)。结论 用于诊断的超声破坏全氟显微泡可诱导HL-7702细胞悬液发生声孔效应。随着辐照时间的延长,声孔效应发生率增加。辐照时间延长至10~20 min时,可出现细胞的不可逆性损伤。应用于诊断目的时,高机械指数超声辐照下,辐照时间越短越好,不宜超过5 min;应用于基因或药物的传输时,则选择辐照时间以5 min为佳,必要是可达10 min。 |
| 英文摘要: |
| Objective To investigate the sonoporation and cell killing effect on human hepatocyte (HL-7702 ) with domestic ultrasound contrast agent (Perfluoropropane-albumin microsphere), induced by ultrasound for different exposure time. Methods Suspensions of hepatocyte with microbubbules were exposed to diagnostic ultrasound at frequency of 2 MHz and MI=1.9. The study included contrast group, which was not exposed to ultrasound, and 5 exposed groups were exposed to ultrasound for 30 s, 1 min, 5 min, 10 min and 20 min respectively. The uptake of fluorescein isothiocyanatedextran (FD500) was observed under fluorescence microscopy and the percentages of sonoporation cells were counted, the cell viability was determined by trypan blue stain immediate after exposure, and apoptosis of cells were detected by flow cytometry, with duble staining of fluorescein isothiocyanate (FITC)-labeled Annexin V/propidium iodide (PI). Results ①Fluorescence stain results: Compared with contrast group, the ratios of sonoporation cells of exposed groups increased significantly (P<0.05). Compared with 1min group, the ratio of 5 min group increased significantly, and that of 10 min group increased significantly compared with 5 min group (P<0.05). While there was no significant different between 10 min group and 20 min group (P>0.05). ②Cell killing effects: compared with the contrast group, the ratios of 10 min and 20 min groups increase significantly (P<0.05). Compared with 5 min group the ratio of 10 min increased significantly, and that of 20 min group increased significantly compared with 10 min group (P<0.05). Conclusion Diagnostic ultrasound and Perfluoropropane-albumin microsphere can induce sonoporation in HL-7702. The ratios of sonporation cells increased with the increase of exposure time. However, the cell killing effect appeared when exposure time prolonged to 10 min and 20 min. For diagnosis, the exposure time should be as short as possible when under high MI, and should better be shorter than 5 min. While for delivering gene and drugs, the exposure time should better be 5 min and could prolong to 10 min when necessary. |
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