| 廖沁,周希瑗,王志刚,李佳.超声微泡造影剂介导PEDF质粒转染大鼠视网膜的实验研究[J].中国医学影像技术,2008,24(8):1149~1152 |
| 超声微泡造影剂介导PEDF质粒转染大鼠视网膜的实验研究 |
| Study on ultrasound-targeted microbubble destruction for PEDF gene delivering to inhibit rat choroidal neovascularization |
| 投稿时间：2008-02-25 修订日期：2008-07-10 |
| DOI： |
| 中文关键词: 超声检查,介入性 造影剂 视网膜色素上皮源性因子 基因治疗 |
| 英文关键词:Ultrasonography, interventional Contrast media Pigment epithelium derived factor Gene therapy |
| 基金项目:国家自然科学基金重点项目(30430230)、国家863计划项目(2006AA02Z4F0)。 |
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| 中文摘要: |
| 目的 探讨超声微泡造影剂介导色素上皮源性因子(PEDF)质粒转染大鼠视网膜、脉络膜的效率及治疗脉络膜新生血管(CNV)效果。 方法 氩绿激光对Long-Evans大鼠视网膜进行光凝建造CNV 模型。将24只造模成功的CNV大鼠分为2 组:①空白组,②超声辐照微泡转染组。于转染后14 d,分别进行眼底荧光血管造影(FFA),RT-PCR 和免疫荧光检查。 结果 转染后14 天超声微泡能介导PEDF 质粒对大鼠视网膜、脉络膜的转染,并且对CNV 有抑制作用。 结论 利用一定能量的超声击碎携带PEDF质粒的超声微泡造影剂,能够有效地提高PEDF质粒在大鼠视网膜、脉络膜的转染效率,对大鼠脉络膜新生血管有一定抑制作用。 |
| 英文摘要: |
| Objective To investigate whether ultrasound-mediated microbubble destruction could effectively deliver PEDF gene to the rat retina and inhibit rat CNV. Methods Twenty-four CNV Long-Evans rats induced by argon laser were randomly divided into two groups: group 1, the rats were accepted no treatment; group 2, microbubbles attached with the naked plasmid DNA of PEDF were infused into the femoral vein of rats with exposed to ultrasound immediately. Rats were killed and eyes were enucleated at 14 days after treatment. Gene and protein expression of PEDF in the rats’ retina and choroids was detected by RT-PCR and immunofluorescence respectively. The effect of PEDF gene transfer on CNV was examined by FFA. Results Fourteen days later, infection efficiency of the group 2 was higher than that of group 1 (P<0.05). With the administration of ultrasound-mediated microbubbles destruction, the CNV were inhibited effectively. Conclusion The PEDF expression in rats’ retina and chorioid was increased due to the administration of ultrasound-mediated microbubbles destruction resulted in CNV being inhibited. |
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