| 项飞翔,王新房,谢明星,周翔,张艳容,张丽.国产超声造影剂在超声辐照下对肝细胞产生的生物效应[J].中国医学影像技术,2007,23(3):325~328 |
| 国产超声造影剂在超声辐照下对肝细胞产生的生物效应 |
| Bioeffect of domestic ultrasound contrast agent on cultured hepatocyte induced by ultrasound |
| 投稿时间：2006-11-14 修订日期：2007-02-12 |
| DOI： |
| 中文关键词: 超声造影剂 肝细胞 声孔效应 细胞溶解 凋亡 |
| 英文关键词:Ultrasound contrast agent Hepatocyte Sonoporation Cell lysis Apoptosis |
| 基金项目: |
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| 中文摘要: |
| 目的 探讨国产超声造影剂全氟显在不同机械指数(MI)超声辐照下对正常肝细胞(HL-7702)的声孔效应及细胞损伤。方法 在不同的MI(分别为0.15、0.61、1.2、1.9)下分别对各组加入全氟显的旋转的HL-7702细胞悬液进行超声辐照,频率2 MHz,持续照射时间10 min。对照组未经超声辐照。应用荧光显微镜观察大分子物质FD 500进入细胞的情况,检测发生声孔效应的百分率,应用台盼蓝染色检测细胞活力,以Annexin Ⅴ-FITC/PI双染并应用流式细胞仪检测细胞凋亡。结果 声孔效应:与对照组比较其余各组声孔效应均有增加,有统计学意义(P<0.05)。随着MI的升高,声孔效应也随之增加,MI为1.2及1.9组与其他各组比较,差异有统计学意义(P<0.05)。细胞损伤:随着MI的升高,细胞损伤 (溶解＋凋亡) 虽有增加,但与对照组相比,仅当MI为1.9时细胞损伤增加的程度才有统计学意义(P<0.05)。结论 应用于诊断的超声可使加入全氟显的HL-7702细胞悬液发生声孔效应。随着MI的升高声孔效应也随之增加;MI≤1.2时,全氟显不引起明显的细胞损伤,当MI=1.9时细胞损伤有统计学意义。 |
| 英文摘要: |
| Objective To investigate the sonoporation and cell killing effect on human hepatocyte (HL-7702) with domestic ultrasound contrast agent (Perfluoropropane-albumin microsphere), induced by ultrasound in different mechanic index (MI). Methods Suspensions of HL-7702 were exposed to diagnostic ultrasound at frequency of 2 MHz and for continuous 10 min, MI=0.15, 0.61, 1.2 and 1.9, while the contrast group did not expose to ultrasound. The uptake of fluorescein isothiocyanatedextran (FD500) by HL-7702 was observed under fluorescence microscopy and the percentages of sonoporation cells were counted, the cell viability was determined by trypan blue stain, and apoptosis of cells were detected by flow cytometry, with double staining of fluorescein isothiocyanate (FITC)-labeled Annexin V/propidium iodide (PI). Results Compared with contrast group, sonoporatin of other groups increased significantly (P<0.05). And sonoporation increased with MI, those of groups of MI=1.2 and 1.9 different significantly with other groups (P<0.05). Cell killing effect, including cell lysis and apoptosis, also increased with MI, but only when MI=1.9, the effect increased significantly (P<0.05). Conclusion Sonoporation were observed in HL-7702 in suspension with the addition of Quanfuxian exposed by diagnostic ultrasound. The level of sonoporation increased with MI. When MI≤1.2, Perfluoropropane-albumin microsphere did not induce cell killing effect significantly, while when MI=1.9, the effect increased significantly. |
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