| 陈志奎,林礼务,王艳,林晓东,林学英,何以敉,高上达,俞丽云.超声介入复方中药"99-克星"活性组分诱导肝癌细胞凋亡及机制[J].中国医学影像技术,2006,22(12):1792~1794 |
| 超声介入复方中药"99-克星"活性组分诱导肝癌细胞凋亡及机制 |
| Induction apoptosis effect of active compound from Chinese traditional medicine "99-star" on liver cancer |
| 投稿时间：2006-09-10 修订日期：2006-11-23 |
| DOI： |
| 中文关键词: 超声 肝肿瘤 中药 细胞凋亡 基因调控 |
| 英文关键词:Ultrasound Liver neoplasms Chinese traditional medicine Apoptosis Gene regulation |
| 基金项目:本课题受福建省科委研究基金资助(2000Z138)。 |
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| 中文摘要: |
| 目的 探讨超声引导瘤内注射用复方中药"99-克星"的活性组分AG-05诱导体外培养肝癌HepG2细胞凋亡作用及相关机制。方法 不同浓度AG-05作用于体外培养肝癌HepG2细胞后,应用荧光显微镜观察细胞形态学改变,流式细胞术分析细胞凋亡率变化,Western blotting方法 检测细胞bcl-2、caspase-3蛋白表达的改变。结果 AG-05作用48 h后肝癌HepG2细胞出现染色质边集、凋亡小体形成等典型的凋亡形态学改变,细胞凋亡率明显升高,10、20 μg/ml组分别为(13.5±2.1)％和(20.1±4.3)％;AG-05具有抑制肝癌细胞bcl-2表达,活化caspase-3的作用,并且呈一定的量效、时效关系。结论 复方中药"99-克星"活性组分AG-05可诱导肝癌细胞凋亡,其作用机理与抑制bcl-2蛋白表达,活化caspase-3有关。 |
| 英文摘要: |
| Objective To probe the effect of active compound (AG-05) from Chinese traditional medicine "99-star" on liver cancer, including induction apoptosis and the reflection mechanism. Methods After treated with AG-05 for 48 h, HepG2 cells morphology was observed under fluorescence microscope, and the flow cytometric analysis was performed to detect apoptosis. The expression of apoptosis-related proteins were detected by Western blotting. Results Visible morphology changed, such as chromatin margination and apoptosis body appeared a portion of HepG2 cells were observed. The flow cytometric analysis showed that apoptosis rates of HepG2 cells treated with AG-05 10, 20 μg/ml were (13.5±2.1)％ and (20.1±4.3)％, respectively. Western blotting showed that the expression of bcl-2 was inhibited significantly, while caspase-3 was activated after AG-05 treatment. Conclusion The above findings indicated that the active compound (AG-05) from Chinese traditional medicine "99-star" could induce HepG2 cells apoptosis obviously. Its major mechanism could be inhibiting bcl-2 and activing caspase-3 expression. |
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