| 朱光宇,滕皋军,牛焕章,郭金和,卢勤,李国昭,余辉,陈晟.兔VX2肝癌模型建立方法的比较及股动脉插管方法的应用[J].中国医学影像技术,2006,22(12):1785~1788 |
| 兔VX2肝癌模型建立方法的比较及股动脉插管方法的应用 |
| Comparison of methods for building rabbit model bearing VX2 liver tumor and application of femoral artery catheterization |
| 投稿时间：2006-07-19 修订日期：2006-09-15 |
| DOI： |
| 中文关键词: 兔 VX2肿瘤 肝肿瘤 放射学,介入性 血管造影术 |
| 英文关键词:Rabbits VX2 tumor Liver neoplasms Radiology, interventional Angiography |
| 基金项目:国家自然科学基金资助项目(30470507)。 |
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| 中文摘要: |
| 目的 比较不同的兔VX2肝癌模型的建立方法 ,探讨股动脉插管技术的应用。方法 将60只实验兔随机分成3组,每组20只,分别用瘤细胞悬液直视下注射法、直视下瘤块注入后用明胶海绵封堵穿刺通道法及直视下瘤块注入后局部压迫法建立兔VX2肝癌模型。结果 60只实验兔中死亡2只,存活兔经剖腹探查证实肝脏种植成功54只(成功率93.1％,54/58);三组成瘤率分别为79％(15/19)、100％(19/19)、100％(20/20),前者成瘤多为多结节、分叶状,腹腔及全身转移多见,与后两者均有统计学差异,后两者成瘤状况无统计学差异,多为孤立病灶且成瘤速度快,腹腔及远处转移少见。成瘤后的影像学及组织病理学亦证实前述结果 。所有实验兔处死前均经股动脉直视下Seldinger法置入4F导管鞘后引入导管行血管造影及其他介入操作,成功56例(成功率96.6%,56/58)。结论 直视下注入瘤块后局部压迫法操作简便、成瘤率高,且多为孤立病灶,转移少见,更适合建立兔VX2肝癌模型的实验要求。经股动脉置鞘后行介入操作方法 可行,尤其适用于复杂的介入操作。 |
| 英文摘要: |
| Objective To compare the advantages and disadvantages of several Methods to establish the rabbit VX2 liver tumor model, and to investigate the application of femoral artery catheterization. Methods Sixty rabbits were randomly divided into 3 groups with 20 for each and rabbit model with VX2 liver tumor was established in each group with different Methods including direct injection with suspension of tumor cell, implanting tumor mass directly with gelatin sponges to block the puncture way or pressing the region locally. Results Two of 60 experimental rabbits died. Fifty-four of the survival were successfully implanted with the VX2 tumor in the liver (success rate: 93.1%, 54/58), which was demonstrated through exploratory laparotomy. The success rate of transplanted tumors in 3 groups was 79% (15/19), 100% (19/19) and 100% (20/20) respectively. The tumor in former group was often multinodular, sublobe, and metastatic in abdominal cavity or all over the body. There was statistical difference between the former and the later two groups. The tumor in the later two groups was always solitary, growing quickly and metastatic rarely in abdominal cavity or distant place, which was proved by radiology and histopathology. There was no statistical difference in the later two groups. 4F catheter sheath was put directly into all of the experimental rabbits through femoral artery with Seldinger's centesis before being killed and then angiography and other interventional operations were performed, which was successful in 56 cases (success rate: 96.6%, 56/58). Conclusion The method of injecting tumor mass directly with local press is easy in operation and shows high successful rate with the result of often forming solitary focus and seldom matastasis, which is more suitable for the experiment of building rabbit model bearing VX2 liver tumor. It is feasible to put the catheter sheath through femoral artery and then make especially complex interventional operation. |
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