| 张秀梅,冯珏,马慧莉,王亚平,王鹏,杨新毅.99Tcm-octreotide在荷H22肝癌小鼠体内分布和显像研究[J].中国医学影像技术,2006,22(6):945~948 |
| 99Tcm-octreotide在荷H22肝癌小鼠体内分布和显像研究 |
| Study of biodistribution and imaging for 99Tcm-octreotide in bearing H22 hepatocarcinoma mice |
| 投稿时间：2005-10-06 修订日期：2005-11-15 |
| DOI： |
| 中文关键词: 生长抑素受体 奥曲肽 核素显像 小鼠 生物分布 |
| 英文关键词:Somatostatin receptor Octreotide Radionuclide imaging Mice Biodistribution |
| 基金项目:本课题受河北省卫生厅基金资助 (05009)。 |
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| 中文摘要: |
| 目的 探讨99Tcm-octreotide作为生长抑素受体阳性肿瘤显像剂的可能性。方法 99Tcm-octreotide的制备采用直接标记奥曲肽冻干品药盒的方法。①体内分布实验:25只正常KM小鼠和15只荷H22肝癌小鼠,静脉注射显像剂0.1 ml(3.7 MBq)后行常规体内分布实验,计算%ID/g及肿瘤的T/NT值。②荷瘤鼠显像:5只荷瘤鼠于注入显像剂后1、2、3、4、6 h和24 h不同时相分别显像并计算T/B值。结果 ①体内分布:正常小鼠体内分布显示,血液在0.5 h放射性最高 ,随后迅速清除。双肾1 h摄取达最高 ,提示显像剂主要经泌尿系统排泄。99Tcm-octreotide在荷瘤鼠的体内分布与正常小鼠一致,肿瘤与其他脏器组织的T/NT较高。②受体显像:注射显像剂1 h后,小鼠全身轮廓清晰,肾和膀胱高度摄取显像剂,荷瘤鼠的已知肿瘤部位呈阳性显像。在2 h肿瘤部位的放射性浓聚最高,显像效果最佳。随后放射性逐渐减弱,但肿瘤影像仍较清晰。 24 h时图像模糊。注射显像剂后1、2、3、4、6 h和24 h的T/B值分别为2.64、4.46、7.25、5.40、5.49和6.48, 2 h以后各时相与1 h相比有显著性差异(P<0.05),但3 h以后与前一时间点比较差异均无显著性(P>0.05)。结论 一步法药盒制备的99Tcm-octreotide,在小鼠H22肝癌组织中分布快,消除较慢,靶和本底比值较高,注射后2h肿瘤显像最佳。99Tcm-octreotide是一种很有前景的生长抑素受体显像剂。 |
| 英文摘要: |
| Objective To evaluate the practicability of 99Tcm-octreotide as an imaging agent of somatostatin receptor-positive tumors. Methods Preparation of 99Tcm-octreotide was that technetium-99m was labeled directly with cold-kit of octreotide. ①Animal biodistribution experiment: Twenty-five normal and fifteen tumor-bearing mice were injected with 99Tcm-octreotide in 0.1 ml containing 3.7 MBq. The biodistribution was performed in routine methods and the percentage of radioactivity administered dose per gram of tissue (%ID/g) and target to non-target ratios (T/NT) at different interval were calculated. ②Imaging of tumor-bearing mice: Five tumor-bearing mice were imaged respectively at 1, 2, 3, 4, 6, 24 h pi and calculated target to background ratios(T/B). Results ①The normal mice biodistribution indicated that the blood radioactivity presented peak at 30 min pi and 99Tcm-octreotide were cleared rapidly thereafter. Physiological uptakes of kidney were the highest at 1 h pi and showed the radio pharmaceutical excreted mostly by kidney. The in vivo distribution of bearing-tumor mice was similar to normal mice. The tumor T/NT were higher and reached to (6.44±0.20) at 2 h pi. ②Receptor scintigraphy showed that the imaging of mice outline were clearly seen at 1 h pi. The radioactivity uptake in kidneys and bladder were very highly too. Known tumor sites showed rapid and intense tracer accumulation. The tumor imaging was high quality at 2 h and then gradually unclear from time to time. Up to 24 h, the imaging was blurred; The T/B were 2.64, 4.46, 7.25, 5.40, 5.49 and 6.48 at 1, 2, 3, 4, 6, 24 h respectively. The T/B was raised rapidly from 1 h to 2h and 3h, and the difference was significant between that of 1 h and other times (P<0.05). After 3 h, the uptake change was not obvious with time. Conclusion The distribution of 99Tcm-octreotide by one step method labeled in bearing-tumor mice is very quickly, extinction is slower relatively and the ratios of tumor to background are high. The imaging of tumor is best quality 2h pi. These results suggest that 99Tcm-octreotide is a promising radiopharmaceutical for somatostatin receptor scintigraphy. |
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