| 戴维德,范智慧,陈敏华,河福金,李洪民.射频消融对大鼠外周血及肝脏局部组织白细胞介素-10水平的影响[J].中国医学影像技术,2006,22(6):808~810 |
| 射频消融对大鼠外周血及肝脏局部组织白细胞介素-10水平的影响 |
| Effect of radiofrequency ablation of normal rat liver on IL-10 in peripheral-blood and local area of liver |
| 投稿时间：2006-02-24 修订日期：2006-05-11 |
| DOI： |
| 中文关键词: 射频消融 大鼠 肝脏 IL-10 |
| 英文关键词:Radiofrequency ablation Rat Liver IL-10 |
| 基金项目:北京市科委重大项目培育专项(Z0005190040431)。 |
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| 中文摘要: |
| 目的 探讨肝脏射频消融前后大鼠外周血及肝脏局部组织白细胞介素-10(Interleukin-10,IL-10)水平的变化及意义。方法 30只健康SD大鼠随机分为射频后1周组、射频后2周组和对照组,每组10只。射频后1周组及射频后2周组分别在射频后1周及2周处死,对照组不做射频即处死,取外周血及射频灶周边0.5 cm范围内肝组织(对照组取正常肝组织),采用Ficoll密度梯度离心法分离外周血及射频灶周边肝组织单个核细胞,应用流式细胞术检测其IL-10表达水平。结果 对照组大鼠的外周血IL-10表达水平为94.8333±3.5388。射频后1周为88.96±5.7230,射频后2周为63.08±13.7723,射频后2周组与对照组比较IL-10表达水平降低,差异有显著性。射频后1周组与对照组比较差异没有显著性。对照组大鼠的肝组织IL-10表达水平为94.9333±2.0817。射频后1周为93.3400±2.1652,射频后2周为72.9400±10.4677,射频后2周组与对照组比较IL-10表达水平降低,差异有显著性。射频后1周组与对照组比较差异没有显著性。结论 射频消融后大鼠外周血及射频灶周边肝组织IL-10表达水平降低,推断RFA可能削弱IL-10对树突状细胞分化成熟的抑制作用。 |
| 英文摘要: |
| Objective To study the effect of radiofrequency ablation of normal rat liver on IL-10 in peripheral-blood and local area of liver. Methods Thirty healthy SD rats were randomly separated into group 1 week after RFA, group 2 weeks after RFA and control group; there were ten rats in each group. Peripheral blood and liver tissue around the local area of control group, group 1 week after RFA and group 2 weeks after RFA were taken out respectively without RFA, 1 week and 2 weeks after RFA. The mononuclear cells of peripheral blood and liver tissue were separated by Ficoll density gradient centrifugation. The expression level of IL-10 in peripheral blood and liver tissue were analyzed with flowcytometry. Results The expression level of IL-10 in peripheral blood of control group was 94.8333±3.5388, and that of group 1 week and 2 weeks after RFA were respectively 88.96±5.7230 and 63.08±13.7723. The difference between control group and group 2 weeks after RFA was marked. The expression level of IL-10 in liver tissue of control group was 94.9333±2.0817, and that of local area around RFA focus of group 1 week and 2 weeks after RFA were respectively 93.3400±2.1652 and 72.9400±10.4677. The difference between control group and group 2 weeks after RFA was marked. Conclusion RFA can reduce the expression level of IL-10 in peripheral-blood together with local area of normal rat liver and may decrease inhibitory action of IL-10 on differentiation and maturation of dendritic cells to a certain extent. |
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