| 曹智刚,郭启勇,马力,何东风,金罡.热化疗对人胆管癌细胞增殖和凋亡的影响[J].中国医学影像技术,2006,22(6):804~807 |
| 热化疗对人胆管癌细胞增殖和凋亡的影响 |
| Effect of thermochemotherapy on the proliferation and apoptosis of human cholangiocarcinoma cell |
| 投稿时间：2006-03-10 修订日期：2006-05-12 |
| DOI： |
| 中文关键词: 胆管 QBC939细胞 热化疗 人 增殖 凋亡 |
| 英文关键词:Bile duct QBC939 cell Thermochemotherapy Human Proliferation Apoptosis |
| 基金项目:本课题受辽宁省科委专用基金资助(00225001)。 |
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| 中文摘要: |
| 目的 探讨表阿霉素(EADM)热化疗对人胆管癌细胞(QBC939)的增殖和凋亡的作用。方法 以体外培养的人胆管癌细胞株为研究对象,采用水浴加热法进行体外细胞毒实验(MTT)、透射电镜观察及流式细胞仪检测,观察热疗、化疗、热化疗对人胆管癌细胞的生长抑制和凋亡的影响。结果 42℃以上单纯热疗对QBC939细胞有明显杀伤作用(P<0.01),42℃以上热化疗对QBC939细胞有明显的协同或相加作用(P<0.01)。透射电镜和流式细胞术均观察到热疗、化疗、热化疗诱导细胞凋亡的作用(P<0.01);热化疗有协同作用(P<0.01)。结论 热疗、化疗、热化疗均抑制QBC939细胞增殖;热疗、化疗、热化疗诱导细胞凋亡为其抗癌机制之一;热疗提高化疗药物的细胞毒作用。 |
| 英文摘要: |
| Objective To study the effect of EADM thermochemotherapy on the proliferation and apoptosis of human cholangiocarcinoma cell. Methods Using QBC939 cell line, to observe the inhibition of cell growth and apoptosis with MTT assay, FCM, transmission electron microscopy under water-heating with EADM. Results Hyperthermia, above the temperature of 42℃ could obviously inhibit the QBC939 cell (P<0.01), and the combined use of hyperthermia and EADM showed evident synergistic or additional effects (P<0.01). Transmission electron microscopy and FCM examination observed apoptosis of QBC939 cell in every group (P<0.01), and remarkable synergistic effect between hyperthermia and the drug (P<0.01). Conclusion Hyperthermia, chemotherapy and thermochemotherapy can all produce a growth inhibiting effect on QBC939 cell. Induction of apoptosis of QBC939 cell can be achieved by hyperthermia and chemotherapy. Hyperthermia in combination with EADM would provide a greater cytotoxic effect on QBC939 cell. |
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