| 刘晟,王维,于小平,毛俊,容鹏飞.MPTP诱导猴偏侧帕金森病模型的制作[J].中国医学影像技术,2006,22(3):353~356 |
| MPTP诱导猴偏侧帕金森病模型的制作 |
| Establishment of hemi-Parkinsonism model of rhesus induced by MPTP |
| 投稿时间：2005-10-22 修订日期：2006-01-05 |
| DOI： |
| 中文关键词: 1-甲基-4-苯基-1,2,3,6-四氢吡啶 恒河猴 帕金森病 正电子发射型计算机断层显像 多巴胺转运蛋白 |
| 英文关键词:1-methyl 4-phenyl tetrahydropyridine Rhesus Parkinson's disease PET DAT |
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| 中文摘要: |
| 目的 探讨偏侧帕金森病恒河猴动物模型的成模率及影像、病理的对比。方法 经一侧颈内动脉注入1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)后,对实验组猴的行为表现及影像学表现:包括MRI、PET及SPECT进行检测及酪氨酸羟化酶(TH)免疫组化染色。结果 注射 MPTP后第5~7天逐渐出现偏侧帕金森病症状,肌注阿朴吗啡后动物向左侧快速旋转;PET检查示实验组猴右侧(患侧)18F-脱氧葡萄糖平均标准摄取值较左侧明显减低,SPECT检查示多巴胺转运蛋白(DAT)显像右侧纹状体放射性摄取明显减低,二者均与对侧及给药前对比统计学上差异有显著性差异(P<0.05)。TH免疫组化染色示右侧黑质多巴胺能神经元明显减少。结论 经一侧颈内动脉注射MPTP制作偏侧帕金森病猴模型安全、简单、成模率高,是研究帕金森病的一种理想的帕金森病动物模型。 |
| 英文摘要: |
| Objective To evaluate the ratio of hemi-Parkinsonism model induced by MPTP and compare with image and pathobiology. Methods After injecting MPTP in right side internal carotid artery, the ability to move was quantified by using a pedometer. The apomorphine evoked rotation was also tested. The metabolism of glucose within the monkey brain was measured by positron emission tomography (PET) using 18F-FDG, and the metabolism of dopamine was also measured by single photon emission computerized tomography (SPECT) using 99Tcm-TRODAT-1. Results Seven days after the injection of MPTP in right side internal carotid artery, the monkeys presented the Parkinson's disease symptoms and left-twirling. Examination of PET and SPECT showed that the radioactivity was much less in right midbrain and striatum than that in the contralateral side, there was significant difference between the pre- and post-operation groups or in right and left groups. Tyrosine hydroxylase positive neurons in the substainial nigra were obviously reduced. Conclusion The establishment of hemi-Parkinsonism model of rhesus induced by MPTP in right side internal carotid artery is a safe and simple way, and it is ideal for the study of Parkinson's disease. |
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