| 孙军辉,滕皋军,居胜红,马占龙,麦筱莉,李爱梅,张宇,马明.磁标记大鼠肾脏骨髓间充质干细胞移植MR成像研究[J].中国医学影像技术,2006,22(2):205~208 |
| 磁标记大鼠肾脏骨髓间充质干细胞移植MR成像研究 |
| MR imaging of implanted magnetically labeled rat bone marrow mesenchymal stem cells in normal kidney |
| 投稿时间：2005-10-18 修订日期：2006-01-10 |
| DOI： |
| 中文关键词: 间充质干细胞 磁共振成像 肾移植 |
| 英文关键词:Mesenchymal stem cells Magnetic resonance imaging Kidney t ransplantation |
| 基金项目:本课题受高等学校博士学科点专项科研基金(20040286037)、东南大学国家自然基金预研项目基金(XJ0490168) 、东南大学优秀博士学位论文基金( YBJJ0518) 资助。 |
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| 中文摘要: |
| 目的 对移植入大鼠正常肾脏的Fe2O3-PLL 标记MSCs 进行MR 成像并探讨其成像技术。方法 分离培养大鼠骨髓MSCs ,Fe2O3-PLL 标记细胞。将标记和未标记细胞经左肾动脉移植入大鼠肾脏,移植后即刻,第1 、3 、5 、8 天应用MRI 对移植细胞进行活体示踪并与肾脏组织切片对照。结果 MSCs 的Fe2O3-PLL 标记率近100 %。标记细胞移植后T2 WI 肾脏皮质区信号强度明显下降,持续至移植后第8 天。组织学分析见绝大多数标记细胞分布于肾小球内。结论 1. 5T 磁共振仪可对移植入正常肾脏的Fe2O3-PLL 标记细胞进行活体成像,T2 WI 信号变化最明显。 |
| 英文摘要: |
| Objective To evaluate in vivo magnetic resonance (MR) imaging with a conventional 1. 5 T system for depic-tion and t racking of int ravascularly injected magnetically labeled bone marrow mesenchymal stem cells (MSCs) in rat normalkidney. Methods Rat bone marrow MSCs were isolated , purified , expanded and then incubated with home synthesizedFe2O3-PLL. Prussian blue stain was employed for identifying int racellular irons. MSCs were injected into renal arteries of 12recipient rats (labeled cells in nine , unlabeled cells in three) . MR images of kidneys were obtained respectively before injec-tion of MSCs , and immediately , 1 , 3 , 5 , and 8 days af ter t ransplantation. MR imaging findings were analyzed , which werecorrelated with histological findings. Results Rat MSCs were successfully labeled , and labeling efficiency was almost100 %. Prussian blue staining of Fe2O3-PLL labeled cells revealed the presence of iron-containing vesicles or endosomes inthe cytoplasm. In normal kidneys , the labeled MSCs were demonst rated as signal intensity loss in renal cortex on T2-weighted MR images. The signal intensity decrease was visualized up to days 8 af ter t ransplantation. Histological analysesshowed that most Prussian blue staining-positive cells were well correlated with the area where a signal intensity loss was ob-served in MRI. Conclusion 1. 5T MR imaging can monitor administered magnetically labeled MSCs in vivo in normal kid-neys , especially on T2-WI. |
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