| 王荣福,刘萌,张春丽,郭凤琴,赵光宇.单次化疗后荷瘤小鼠肿瘤细胞凋亡显像研究[J].中国医学影像技术,2005,21(11):1667~1670 |
| 单次化疗后荷瘤小鼠肿瘤细胞凋亡显像研究 |
| Study on mice tumor-bearing cell apoptosis imaging in vivo after a single dose of chemotherapy |
| 投稿时间：2005-09-26 修订日期：2005-10-09 |
| DOI： |
| 中文关键词: 细胞凋亡 99Tcm-HYNIC-Annexin Ⅴ 放射性核素显像 荷瘤小鼠 生物分布 |
| 英文关键词:Apoptosis 99Tcm-HYNIC-Annexin Ⅴ Radionuclide imaging Tumor-bearing mice Biodistribution |
| 基金项目:国家自然科学基金资助项目(10275003)。 |
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| 中文摘要: |
| 目的 探讨活体肿瘤细胞凋亡监测作为评价肿瘤对化疗反应的一种新方法的可行性。方法 细胞凋亡分子探针99Tcm-HYNIC-Annexin Ⅴ经化学和放射化学合成获得。20~25 g昆明种小白鼠右前腋下皮下组织接种S-180肉瘤,建立荷肿瘤小鼠模型。荷瘤小鼠在环磷酰胺腹腔内给药化疗8 h、24 h、48 h、72 h后,分别尾静脉注射99Tcm-HYNIC-Annexin Ⅴ,1 h后进行单光子 |
| 英文摘要: |
| Objective To investigate the effectiveness and feasibility of in vivo cell apoptosis imaging of tumor response after a single dose of chemotherapy. Methods 99Tcm-HYNIC-Annexin Ⅴ as cell apoptosis molecular probe was obtained through chemical and radiochemical synthesis. Seven days after being inoculated with S-180 sarcoma in the right upper limbs, the mice weighed 20-25 g were randomized to receive a single dose of cyclophosphamide (150 mg/kg intraperitoneally) as trial groups or to receive the same volume of physiological saline as controls. Having been received cyclophosphamide 8 h, 24 h, 48 h and 72 h later, the mice were injected 99Tcm-HYNIC-Annexin Ⅴ by tail vein. Imaging and radioactivity in tissues were obtained at 1 h postinjection of molecular probe. 99Tcm-DTPA-HSA was used as a marker of tumor blood flow. All Results of radioactivity in tissues were analyzed by the statistic software of SPSS 10.0. Results Cyclophosphamide treatment significantly increased the uptake (percentage activity of injected dose per gram of tissue ) of 99Tcm-HYNIC-Annexin Ⅴ in tumor. The optimum imaging time after a single dose of chemotherapy could be considered at 72 h postinjection ( %ID/g for treated 72 h mice tumor-bearing and %ID/g for controls, P<0.05). The ratio ( %ID/g) of tumor/muscle was higher than those of tumor/blood ( %ID/g) and controls with significant difference (P<0.05). 99Tcm-DTPA-HSA uptake was not significantly different in the treated and untreated groups, which indicated the increased accumulation of 99Tcm-HYNIC-Annexin Ⅴ in the tumor is not attributable to changes in blood flow. Conclusion The present study shows that the radioactive uptake of cell apoptosis in mice tumor-bearing is significantly increased at 72 h after a single dose of cyclophosphamide treatment. The current Results suggest the potential utility of cell apoptosis imaging in vivo as a noninvasive means to assess tumor response after a single dose of chemotherapy. |
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