| 徐爱民,张树辉,林川,李国栋,程红岩,吴孟超.载VEGF-ASODNPLGA纳米粒抑制大鼠肝癌新生血管生成的作用[J].中国医学影像技术,2005,21(11):1659~1662 |
| 载VEGF-ASODNPLGA纳米粒抑制大鼠肝癌新生血管生成的作用 |
| Anti-angiogenesis effectiveness of VEGF antisense oligodeoxynucleotide loaded polylactic-co-glycolic acid nanoparticles in the treatment of Walker-256 hepatoma in rats |
| 投稿时间：2005-07-30 修订日期：2005-09-15 |
| DOI： |
| 中文关键词: 肝肿瘤 抗血管生成 血管内皮生长因子 反义寡核苷酸 微血管密度 纳米粒 聚乳酸-聚乙醇酸共聚物 |
| 英文关键词:Liver neoplasms Anti-angiogenesis Vascular endothelial growth factor Antisense oligodeoxynuclieotide Microvessel density Nanoparticle Polylactic-co-glycolic acid |
| 基金项目:国家自然科学基金面上项目(30471993);上海市科技发展基金(03ZR14030)。 |
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| 中文摘要: |
| 目的 研究VEGF-ASODN PLGA纳米粒对大鼠肝癌血管生成的抑制作用。方法 建立大鼠Walker-256肝癌模型。48只大鼠随机分为4组,均采用肝动脉插管方法注射药物。A组生理盐水组,B组PLGA组,C组单纯反义ODN组,D组VEGF-ASODN PLGA纳米粒组。每组3只大鼠于治疗后1周行组织学及免疫组化检查,以抗CD34单克隆抗体显示血管内皮细胞,根据CD34阳性血管内皮细胞记数来测定肿瘤微血管密度(MVD)。结果 常规病理检查,A、B组组织形态学相似, |
| 英文摘要: |
| Objective To study the anti-angiogenesis effectiveness by using VEGF-ASODN loaded PLGA nanoparticles in the treatment of Walker-256 hepatoma in rats. Methods The planted Walker-256 hepatoma model were established in 48 rats. We examined the therapeutic effectiveness by using VEGF-ASODN loaded PLGA nanoparticles on planted rat models via hepatic artery infusion. Four treatment groups were compared with 12 rats in each group: ① Normal saline (group A); ② Nothing loaded PLGA (group B); ③ AS-ODN (group C); ④ VEGF-ASODN loaded PLGA nanoparticles (group D). Microvessel density (MVD) was evaluated with immunostained by anti-CD34 antibody in 3 rats for each group. Results Microscopically, the morphological features in group A was similar with that of group B. Tumors in group C is often characterized by reduced tumor nests, karyopyknosis and snip necrosis in some tumor cells. Compared with group C, the karyopyknosis and the karyorrhexis of the tumor cells were obvious increased in group D. Furthermore, the chromatin condensation, the contraction, the snip and focal necrosis of tumor cells were also demonstrated. Compared with other three groups, microvessel density (MVD) was significantly reduced in group D (18.1±6.16, P<0.01). Conclusion Transarterial infusion of VEGF-ASODN loaded PLGA nanoparticles may significantly enhance the anti-angiogenesis effect in comparison with using ASODN alone in the treatment of Walker-256 hepatoma in rats. |
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