| 李康安,张锋,马勇杰,张贵祥,古宏晨,胡运胜,赵京龙,李玉洁.SPIO分子探针标记裸鼠肺腺癌移植瘤的磁共振成像及病理学初步研究[J].中国医学影像技术,2005,21(11):1655~1658 |
| SPIO分子探针标记裸鼠肺腺癌移植瘤的磁共振成像及病理学初步研究 |
| In vivo MR imaging and pathology study in mice model labeled with SPIO |
| 投稿时间：2005-07-29 修订日期：2005-09-10 |
| DOI： |
| 中文关键词: 磁共振成像 肺肿瘤 腺癌 超顺磁性氧化铁 |
| 英文关键词:Magnetic resonance imaging Lung neoplasms Adenocarcinoma Superparamagnetic iron oxide |
| 基金项目:本课题受国家自然科学基金资助(30170286)。 |
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| 中文摘要: |
| 目的 建立应用超顺磁性氧化铁(SPIO)分子探针氨基硅烷Fe3O4纳米颗粒标记人肺腺癌的裸鼠移植瘤模型,行磁共振成像和病理观察,探讨其临床价值。方法 制备氨基硅烷Fe3O4纳米颗粒,分别将标记和未标记肺腺癌细胞株植入裸鼠皮下,观察其成瘤情况,行MR扫描和病理观察。结果 标记及未标记纳米分子探针SPIO的肿瘤模型均成功建立,其中前者肿瘤较后者在MR成像中信号强度有明显变化,在T1 |
| 英文摘要: |
| Objective To establish mice model bearing transplanted human lung adenocarcinoma labeled with ultrasmall superparamagnetic iron oxide (SPIO), and to obtain the MR imaging and pathology results. Methods Aminosilane-coated magnetic particles were produced. Both human lung adenocarcinoma cell line labeling with SPIO or not were planted under the skin of mice, respectively. The development of the tumor was observed, then the MR imaging and pathology Results were obtained. Results All the experimental mice have been made transplanted tumor models labeled with SPIO or not. The signal intensity decrease for the labeled tumors with SPIO compared with that for unlabeled tumors was demonstrated on T1WI, T2WI, and FGR/20° and FGR/70° sequences. The percentage change in FGR/20° sequence was the largest. The iron intracellular or in the necrosis could be observed clearly with Prussian blue stain. Conclusion The mice models bearing transplanted human lung adenocarcinoma labeling with aminosilane-coated nano Fe3O4 particles are stable, and they can be observed with 1.5T MR equipment in vivo. |
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