| 李康安,马勇杰,张贵祥,古宏晨,张锋,胡运胜,赵京龙,李玉洁.磁共振对SPIO标记人肺腺癌细胞成像初步研究[J].中国医学影像技术,2005,21(11):1652~1654 |
| 磁共振对SPIO标记人肺腺癌细胞成像初步研究 |
| MR study on SPIO labeling human lung adenocarcinoma cell line SPC-A-1 in vitro |
| 投稿时间：2005-07-30 修订日期：2005-09-10 |
| DOI： |
| 中文关键词: 磁共振成像 肺肿瘤 腺癌 超顺磁性氧化铁 |
| 英文关键词:Magnetic resonance imaging Lung neoplasms Adenocarcinoma Superparamagnetic iron oxide |
| 基金项目:本课题受国家自然科学基金资助(30170286)。 |
|
| 摘要点击次数: 2540 |
| 全文下载次数: 1302 |
| 中文摘要: |
| 目的 探讨磁共振对超顺磁性氧化铁(SPIO)氨基硅烷Fe3O4纳米颗粒体外标记人肺腺癌(SPC-A-1)进行成像的可行性。方法 制备氨基硅烷Fe3O4纳米颗粒,分别培养肺腺癌细胞株SPC-A-1及人胚肺细胞株WI38至对数生长期,更换为分散有氨基硅烷Fe3O4纳米颗粒的培养液,经培养1、3、6 h,分别取所培养的细胞进行电镜超微结构观察和磁共振T1 |
| 英文摘要: |
| Objective To study the feasibility of MR imaging for labeled human lung adenocarcinoma cell line (SPC-A-1) with ultrasmall superparamagnetic iron oxide (SPIO) particles in vitro. Methods Both human lung adenocarcinoma cell line SPC-A-1 and human lung embryo cells WI38 were cultured respectively in medium contained SPIO (aminosilane-coated nano Fe3O4 particles). From one to six hours, the ultrastructure of both cells was observed by transmission electron microscopy to determine particles uptake and their distribution in cells. The cells underwent MR imaging with T1WI, T2WI and FGR/30° sequences. Results Human lung adenocarcinoma cell line SPC-A-1 have taken up a lot of SPIO particles within the first 6 hours, while normal human lung embryo cells WI38 do not take up any nanoparticles under the same conditions. The signal intensity decrease for the labeled cells compared with that for unlabeled cells was demonstrated on T1WI, T2WI and FGR/30° sequences. The percentage change in FGR/30° sequence was the largest. The percentage change in signal intensity was larger for 1×108 labeled cells than that for 5×107 labeled cells. Conclusion Human lung adenocarcinoma cell line SPC-A-1 can be labeled with SPIO particles, and the labeled cells can be imaged with 1.5T MR equipment. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
