| 李玉洁,张贵祥,赵国旗,胡运胜,赵京龙,周根泉.颌面部VX2肿瘤DCE-MRI与乏氧程度及VEGF表达的相关性研究[J].中国医学影像技术,2005,21(11):1644~1647 |
| 颌面部VX2肿瘤DCE-MRI与乏氧程度及VEGF表达的相关性研究 |
| DCE-MRI study of maxillofacial VX2 tumors and its relationship between tumor hypoxia and VEGF expression |
| 投稿时间：2005-06-20 修订日期：2005-08-20 |
| DOI： |
| 中文关键词: 磁共振成像 图像增强 VX2肿瘤 乏氧 血管内皮生长因子 |
| 英文关键词:Magnetic resonance imaging Image enchancement VX2 tumor Hypoxia Vascular endothelial growth factor |
| 基金项目:本课题受国家自然科学基金资助(30170286)和上海市卫生局科学技术发展基金资助(01436)。 |
|
| 摘要点击次数: 2535 |
| 全文下载次数: 904 |
| 中文摘要: |
| 目的 分析颌面部VX2肿瘤DCE-MRI动态曲线与肿瘤乏氧染色和血管内皮生长因子(VEGF)表达水平的相关性,探讨DCE-MRI在肿瘤乏氧评价中的价值。方法 8只新西兰大白兔于单侧颌面部咬肌内接种VX2肿瘤细胞0.5 ml,接种后10天开始,每隔2天取一只荷瘤兔行DCE-MRI检查,绘制肿瘤动态增强曲线,计算最大强化率(MER)和强化率斜率(SLE)值。对处死的荷瘤兔,取对应层面病理标本行HE染色、乏氧染色和VEGF染色分析。结果 8只实验兔成瘤率达100％,肿瘤 |
| 英文摘要: |
| Objective To analyze the correlation of dynamic contrast-enchanced MR imaging (DCE-MRI) with tumor hypoxia and vascular endothelial growth factor (VEGF) expression and to evaluate the value of DCE-MRI in tumor hypoxia imaging. Methods Eight New Zealand rabbits were inoculated with 0.5 ml (1×108/ml) VX2 tumor cells in the maxillofacial masseter. DCE-MRI was performed every 2 days since the 10th day on. The maximal enhancement ratio (MER) and slope of enhancement (SLE) were calculated. After DCE-MRI, the rabbit was sacrificed and the corresponding tumor specimen was harvested. HE staining, hypoxia and VEGF immunohistochemical staining were carried out. Results All the 8 rabbits had tumors growing. The masses located in the unilateral masseters, and had relatively clear margins. MER had a negative correlation with tumor hypoxia degree (P<0.01). SLE bore a positive correlation with VEGF immunohistochemical score (P<0.05). We compared the continuous sections of hypoxia with VEGF staining of the tumor, and found that where the more hypoxia staining positive, the stronger VEGF expression. But VEGF staining was not always coincident with the staining of hypoxia. Conclusion Tumor hypoxia had a close relationship with the expression of VEGF, and VEGF could promote tumor angiogenesis. DCE-MRI had a good correlation with tumor hypoxia degree and VEGF expression. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
