| 吴文娟,崔慧先,田庆波,刘禧,郭瑞军,李海涛,杨春,邹英华.超声造影评价肝肿瘤血管形成的实验研究[J].中国医学影像技术,2005,21(5):673~676 |
| 超声造影评价肝肿瘤血管形成的实验研究 |
| Experimenal study on liver tumor angiogenesis with contrast-enhanced sonography |
| 投稿时间：2004-09-07 修订日期：2005-01-17 |
| DOI： |
| 中文关键词: 肝肿瘤 VX2肿瘤 兔 超声造影 琼脂糖 微血管密度 |
| 英文关键词:Liver tumor VX2 tumor Rabbit Contrast-enhanced ultrasonography Agarose Microvessel density |
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| 中文摘要: |
| 目的 应用二维超声观测兔VX2肝肿瘤生长及能量多普勒超声造影评价肿瘤血管形成。方法 将VX2瘤组织块悬液0.3ml (约含107~108个瘤细胞/ml) 超声引导下注入15只新西兰白兔肝左叶,拔针前针道注入0.2 ml加热的琼脂糖,超声监测肿瘤生长。肿瘤生长至3周时,静脉团注超声造影剂,能量多普勒检测肿瘤血流信号,并通过图像分析技术定量测定肿瘤内彩色血管平均密度(MCVD)。同时二维超声测定肿瘤大小与大体病理的测定结果进行相关性分析。采用免疫组化技术检测肿瘤内微血管密度(MVD)。结果 ①二维超声肿瘤表现为等回声、低回声及略高回声,以等回声居多,周围可见声晕;②彩色多普勒和能量多普勒均可检出血流信号;③肿瘤与肝实质无明显边界,可见多个核分裂相;④超声与大体病理下测定肿瘤的最大切面直径有相关关系;⑤能量多普勒超声造影测定肿瘤内MCVD与免疫组化测定的肿瘤内MVD之间有线性相关。结论 超声能有效监测肿瘤生长。能量多普勒超声造影测定肿瘤内MCVD,可用于肿瘤内血管形成的评价。 |
| 英文摘要: |
| Objective To evaluate the tumor growth by 2-dimensional ultrasound and the tumor angiogenesis by contrast-enhanced power Doppler. Methods Under the guidance of ultrasound, 0.2 ml of heated liquid agarose was injected to seal the aperture after injection of VX2 tumor fragment suspension 0.3 ml (107-108 cells/ml) into left lobe of the liver for fifteen New Zealand white rabbits. Tumor growth was monitored by ultrasound. Three weeks after implanting VX2 tumor into liver, microbubble contrast agent intravenous bolus injection, blood flow signals were detected by power Doppler and mean color vessel density (MCVD) was calculated for every tumor. Biological features of tumors were examined by routine histology and the value of microvessel density (MVD) of the tumor tissues was examined by immunohistochemistry. Results ①Under the ultrasound, most tumors were isoechoic, some tumors were hypoechoic or slightly hyperechoic, with surrounding echo halo. ②In all the tumors, blood flow was detected by color Doppler and power Doppler. ③The tumors were poorly demarcated from the surrounding liver parenchyma. Multiple mitotic figures were detected on histology. ④The maximum tumor diameters measured by ultrasound and macroscopic pathology were closely correlated. ⑤There was linear correlation between MCVD and MVD of the tumors. Conclusion Ultrasonography is useful in evaluating liver tumor growth. Contrast-enhanced power Doppler can be used for evaluating tumor angiogenesis in vivo. |
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