| 岳殿超,徐辉雄,吕明德,汤庆,李宝金,向邦德.声诺维(SonoVue)增强超声辐照对HepG2细胞膜通透性的影响[J].中国医学影像技术,2005,21(4):510~512 |
| 声诺维(SonoVue)增强超声辐照对HepG2细胞膜通透性的影响 |
| HepG2 membrane permeability effected by SonoVue enhanced ultrasound exposure |
| 投稿时间：2005-03-21 修订日期：2005-04-10 |
| DOI： |
| 中文关键词: 超声造影剂,微泡 空化效应 癌,肝细胞 膜通透性 |
| 英文关键词:Ultrasound contrast agents, microbubbles Cavitation Carcinoma hepatocelluar Membrane permeability |
| 基金项目: |
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| 中文摘要: |
| 目的 探讨超声辐照对人肝癌细胞株HepG2细胞通透性的影响以及声学造影剂声诺维(SonoVue)的增强作用。方法 用连续多普勒超声辐照人肝癌细胞株HepG2,超声发射频率1.9 MHz,软组织热指数(TIS)为0.8,增益设定为80%,输出声强(ⅠSATA)为80.0 mW/cm2,辐照时间均持续60 s。照射时添加或不添加超声造影剂声诺维(SonoVue)。用流式细胞仪检测细胞荧光染色阳性的阳性率。结果 荧光染色阳性细胞的百分率:无超声辐照组或单纯添加SonoVue组分别为(3.06±1.57)%和(1.4±0.04)%,经超声辐照后为(23.72±14.4)%,添加SonoVue辐照后显著增加至(54.78±43.6)%(均P＜0.05)。结论 超声辐照能改变HepG2细胞膜通透性,添加超声造影剂后能显著增强这种作用,有希望成为肿瘤分子生物学治疗的介导手段。 |
| 英文摘要: |
| Objective To explore the role of ultrasound radiation on the permeability of HepG2 cell with/without the intervention of ultrasound contrast SonoVue. Methods HepG2 cell suspension infused with Fluorescent dextran molecules (FD500) was exposed to CW Doppler ultrasound, 1.9 MHz, Tis 0.8, gain 80%,ⅠSATA 80.0 mW/cm2, with/without SonoVue infused. Flow cytometry was used to detect the fluorescent rate. Results The uptake rate of fluorescent cells in control groups (pseudo-US exposure group and only with micro-bubble contrast agent group) were (3.06±1.57)% and (1.4±0.04)% respectively. Significant uptake increases were found in the US exposure group ( %) and US with micro-bubble contrast group ( %). Conclusion Diagnostic ultrasound destruction of contrast-agent microbubbles can enhance the permeability of HepG2 cell membrane, which contributes to the delivery of large molecules. It maybe become a promising biomolecular therapeutic method on oncology. |
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