| 潘长穿,陈文卫,石华,郭瑞强,孙有刚.低频超声对Molt-4肿瘤细胞的生物学效应[J].中国医学影像技术,2004,20(11):1670~1672 |
| 低频超声对Molt-4肿瘤细胞的生物学效应 |
| Bio-effects of Molt-4 cells by low frequency ultrasound |
| 投稿时间：2004-04-26 |
| DOI： |
| 中文关键词: 低频超声 细胞凋亡 肿瘤治疗 |
| 英文关键词:Low frequency ultrasound Apoptosis: Cancer therapy |
| 基金项目: |
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| 中文摘要: |
| 目的 研究低频超声对Molt-4肿瘤细胞的生物学效应,评价低频超声在肿瘤治疗中的应用价值。方法 应用流式细胞术和共聚焦显微镜技术研究低频超声(20 kHz, 0.2 W/cm2)持续辐照Molt-4肿瘤细胞1分钟后的细胞凋亡率和细胞凋亡形态学改变,以及细胞凋亡增殖比(APR)的变化。结果 对照和辐照组细胞凋亡率分别为(2.56±0.61)%和(11.06±1.99)%(P<0.01)。细胞凋亡具有特征性形态学改变,APR上调。结论 低频超声在体外可诱导Molt-4肿瘤细胞凋亡和抑制肿瘤细胞增殖,具有治疗肿瘤潜力。 |
| 英文摘要: |
| Objective To observe the bio-effects of Molt-4 cells treated with low frequency ultrasound, and to study the feasibility of cancer treatment by low frequency ultrasound. Methods The changes of percentage and morphology of apoptosis and ratio of apoptosis to proliferation (APR) after ultrasonic exposure (20 kHz, 0.2 W/cm2, 1 min) were analyzed with FCM and confocal imaging. Results The percentage of apoptosis in control group (n=8) and interventional group (n=8) was (2.56±0.61)% and (11.06±1.99)%, respectively (P<0.01). The apoptic cells had characteristic changes in morphology by confocal. The ratio of apoptosis to proliferation was up-regulated. Conclusion Low frequency ultrasound may offer conference of cancer therapy with its capacity of inducing cancer cells' apoptosis and inhibiting its proliferation in vitro. |
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