| 郑元义,王志刚,冉海涛,张群霞,李晓东,凌智瑜,卢岷,任红.诊断超声加微泡造影剂对新生血管的作用[J].中国医学影像技术,2004,20(3):349~351 |
| 诊断超声加微泡造影剂对新生血管的作用 |
| Diagnostic ultrasound and contrast microbubbles on new capillaries |
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| DOI： |
| 中文关键词: 超声检查 微泡 肿瘤 新生血管 |
| 英文关键词:Ultrasonography Microbubbles Neoplasm New capillary |
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| 中文摘要: |
| 目的 评价诊断超声加微泡造影剂对兔VX2肝肿瘤新生血管的作用。方法 用二维灰阶超声监测6只大白兔肿瘤生长情况,待肿瘤长至长径约0.6~1.0 cm时进行实验。实验分为两组,处理组注射微泡造影剂(全氟显),对照组注射相同剂量的生理盐水。先用二维超声定位肿瘤,然后对处理组大白兔通过兔耳缘静脉缓慢注射微泡造影剂,剂量为0.10 ml/kg,注射时间约5 s。注射的同时行持续性二维超声显像(将MI调至1.4)。整个显像过程持续6~7 min。然后两组肝肿瘤取材后送做透射电镜及切片HE染色检查。结果 超声微泡造影剂能够显著增强肿瘤显像,同时也存在对肿瘤血管明显的生物学效应。在光镜下未见明显的肿瘤血管损坏,未见红细胞渗出等改变,但在电镜下可见肿瘤新生毛细血管明显的超微结构破坏,包括线粒体肿涨、髓样变及胞质空化等改变。结论 高机械指数诊断超声加超声微泡造影剂能够破坏肿瘤新生血管的超微结构。 |
| 英文摘要: |
| Objective To observe the effects of diagnostic ultrasound and contrast microbubbles on the new capillaries of hepatic VX2 tumors in rabbits. Methods Tumor growth rates of six rabbits were observed by B-mode sonography. When the tumors reached 0.6 cm to 1.0 cm in diameter, all the rabbits were used in research. The rabbits were divided into two groups-treatment group and control group. The rabbits in treatment group were injected with contrast microbubbles, while the rabbits in control group were injected with the same dose of physiological salt solution. First we found the place of the tumor by ultrasound imaging, then contrast microbubbles (Optison) at the dose of 0.10 ml/kg were injected into the vascular system of rabbits through the ear veins. At the same time, the tumors were observed by B-mode sonography at a mechanical index (MI) of 1.4. The whole process of imaging lasted about six to seven minutes. After that, the tumors were quickly cut off for electronic microscope and light micrscope (HE stain) observations. Results Contrast microbubbles can significantly enhance tumors sonography, and they also have bioeffects on the new capillayies of tumors. There were no significantly changes in light microscope images, but signicantly ultrastructure changes in the endocells of the capillaries were found in electronic microscope images, such as: swellen mitochondria, myelin figure, and cavilizition of cytoplasm etc. Conclusion Diagnostic ultrasound at high mechanical index plus contrast microbubbles can cause capillaries' ultrastructure changes in tumors. |
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