| 程 文,张青萍,贡雪灏,瘳晓峰.超声监护下免疫抑制大鼠肝癌模型的建立[J].中国医学影像技术,2003,19(7): |
| 超声监护下免疫抑制大鼠肝癌模型的建立 |
| Establishment of Liver Cancer Model from CBRH-7919 Rat Liver Cancer Cell Line in Immune-Suppressed Rats Monitored by Ultrasound |
| 投稿时间：2002-11-03 |
| DOI： |
| 中文关键词: 免疫抑制 肝细胞癌 大鼠 动物模型 超声 |
| 英文关键词:Immunosuppression Hepatocellular carcinoma Rat Animal m |
| 基金项目: |
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| 中文摘要: |
| 目的 用超声监测免疫抑制大鼠肝癌模型的建立情况。方法 将CBRH-7919大鼠肝癌细胞注入经60Co照射和氢化可的松联合免疫抑制的1只大鼠皮下,2周后将生成的肿块接种于免疫抑制的20只大鼠肝内。超声下观察肝内肿块的灰阶和彩色多普勒
表现。2周后处死动物,并对肝内肿块做常规病理检查。结果 3只肝
内接种的大鼠在实验中死亡,余17只接种2周后超声检查均可见肝内肿块生成,肿块直径平均为(1.02±0.15)cm。大鼠肝癌原位模型成功率为85%。病理证实肿块均为肝细胞癌。结论 通过
免疫抑制可建立大鼠CBRH-7919肝细胞癌模型,超声可监测此模型的生长状况。这为肝癌影
像学诊断和局部治疗提供了一种较理想的肝细胞癌模型。 |
| 英文摘要: |
| Objective To monitor the establishment of liver cancer model in immune-suppressed rats with ultrasound.MethodsThe immune system of one rat was inhibited by 60Co irradiation and intraperitoneal injection of Hydrocortisone,and the rat received a subcutaneous inoculation of CBRH-7919 rat liver cancer cells.After 2 weeks' growth,the tumor was transplanted into left liver of other 20 immune-suppressed rats.Then the sonographic and color Doppler features of the tumors were observed by ultrasound.About 2 weeks after the transplantation,the rats were killed and liver tumors were examined pathologically.Results Three out of the 20 rats died during the experiment.About 2 weeks after tumor transplantation,ultrasonography demonstrated that liver tumors grew in 17 rats,and the average diameter of liver tumors was (1.02±0.15)cm,thus the tumor-take rate was 85%.The pathological examination showed that the tumors were hepatocellular carcinoma.ConclusionA rat liver cancer model has been established by immune-suppression and monitored by ultrasound,and may used as an ideal animal model for diagnosis and local therapy of liver cancer. |
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