| 王文,段云友,吕发勤,赵柏山,张龙方,贾化平.超声引导下组织块接种制作兔肾VX2瘤模型及传代保存[J].中国医学影像技术,2002,18(7):616~618 |
| 超声引导下组织块接种制作兔肾VX2瘤模型及传代保存 |
| Modeling and Culture Transfer of Rabbit Renal VX2 Tumor with Ultrasonography-Guided Implantation of Constitution Mass |
| 投稿时间：2002-03-12 |
| DOI： |
| 中文关键词: 肾肿瘤 VX2瘤 兔 动物模型 |
| 英文关键词:Renal tumor VX2 tumor Rabbit Animal model |
| 基金项目:陕西省科学基金资助 (2001SM47)。 |
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| 中文摘要: |
| 目的 探讨术中组织块包埋和超声引导下穿刺组织块接种制作兔肾VX2瘤模型的方法,并对这两种方法进行比较。方法 采用剪切法制备兔VX2瘤组织块悬液,应用超声引导下经皮组织块推注法建立58只兔肾VX2肿瘤模型,应用术中组织块包埋法建立8只兔肾VX2肿瘤模型,应用自然组织谐波技术观察肿瘤生长,并观察浅表淋巴结转移及动物的一般情况;3周内自然死亡者立即行尸体解剖,未死者种植后3周处死行尸体解剖,获得兔肾VX2肿瘤标本,进行HE切片染色。结果 两种方法建立的兔肾VX2瘤模型组织学表现与传代种兔相同;术中包埋组种植成功率50%,超声引导下穿刺组种植成功率96.55%;术中包埋组腹壁种植率25%,超声引导下穿刺组腹壁种植率5.17%。结论 超声引导下穿刺法建立兔肾VX2肿瘤模型方法简便,成功率高,更适用于各种研究的需要。 |
| 英文摘要: |
| Purpose To compare the modeling of rabbit renal VX2 tumor with the intraoparative mass embedding with ultrasonography-guided implantation of constitution mass. Methods To produce constitution mass by shearing. Fifty-eight models of rabbit renal VX2 tumor were set up by ultrasonography-guided implantation of constitution mass, while eight by intraoparative mass embedding. The second harmonic imaging was applied to observe the rabbit renal VX2 tumor and superficial lymph nodes metastasis. The models that died within three weeks were necropsied. The survivals beyond three weeks were put to death and necropsied. The section preparations were stained with HE method. Results The histological manifestation of the modeling of rabbit renal VX2 tumor with these two methods was identical to that of study. The implanative success rate of ultrasonography-guided was 96.55%, while that of the intraoparative mass embedding was 50%. The abdominal wall implantation rate of ultrasonography-guided was 5.17%, while that of the intraoparative mass embedding was 33.33%. Conclusion The modeling of rabbit renal VX2 tumor with ultrasonography-guided implantation of constitution mass is more convenient and successful, therefore more appropriate to the needs of various researches. |
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