袁勋,杜之渝.载单宁酸铁和紫杉醇的相变型纳米粒超声显像与治疗的体外实验研究[J].中国医学影像技术,2020,36(6):
载单宁酸铁和紫杉醇的相变型纳米粒超声显像与治疗的体外实验研究
In vitro study of phasechangeable nanoparticles loaded with Fe-tannic acid/paclitaxelon in ultrasound imaging and treatment
投稿时间:2019-12-13  修订日期:2020-06-24
DOI:
中文关键词:  视网膜母细胞瘤  纳米粒  光热治疗  超声分子显像
英文关键词:Retinoblastoma  Nanoparticles  Photothermal therapy  Ultrasound imaging
基金项目:重庆市科学技术委员会基础与前沿项目
作者单位E-mail
袁勋 重庆医科大学附属第二医院 1799774857@qq.com 
杜之渝* 重庆医科大学附属第二医院 dr.duzhiyu@163.com 
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中文摘要:
      目的 制备一种早期诊断和治疗视网膜母细胞瘤的纳米粒,并体外评价其超声显像和光热治疗效果。方法 制备载单宁酸铁(FeTA)和紫杉醇(PTX)并包裹全氟戊烷(PFP)的PLGA纳米粒,检测其粒径、电位、电镜、吸光度、包封率、载药量等相关表征,评估808激光(1W/cm2,5min)辐照前后的温度变化和超声信号的改变,并于光镜下观察纳米粒相变情况。体外检测FeTA/PLGA/PFP纳米粒的生物安全性。依据不同纳米粒设立空白对照组、激光组、 FeTA/PLGA/PTX/PFP(FPTP)组、FeTA/PLGA/PTX/PFP+激光(FPTP+激光)组,以及不同浓度(0、0.125、0.25、0.5、1.0mg/ml)的FPTP+激光组,检测激光辐照5min后,各纳米粒组对肿瘤细胞的杀伤效果。结果 成功制备出FeTA/PLGA/PTX/PFP纳米粒,其粒径为(155.8±55.68)nm,电位为(-27.3±5.14)mV,透射电子显微镜下可见黑色FeTA外壳包裹灰白核心的球形结构,PTX的包封率为(70.89±8.03)% ,载药率为(9.61±0.63)%。体外激光辐照FPTP纳米粒,温度上升且与浓度呈正相关,B模式和造影模式的超声信号也随浓度增加而增强。体外细胞实验结果表示,FPTP+激光组比空白对照组、激光组、FPTP组对Y79细胞的杀伤作用更强,并随浓度增加而增强。结论 成功制备载单宁酸铁和紫杉醇的相变型纳米粒;该纳米粒具有良好的光热效应和超声显像效果,对Y79细胞有强杀伤作用,有望实现对RB的诊疗一体。
英文摘要:
      Objective This study aimed to assess a nanoparticle for early diagnosis and treatment of retinoblastoma (RB) and explore the ultrasound imaging capability and photothermal effect in vitro. Methods The nanoparticles containing polylacticSacidS(PLGA) shell, Fe-tannic acid (FeTA), paclitaxel (PTX) and perflenapent (PFP) were prepared by double emulsification. In addition, the particle size, potential, encapsulation efficiency and drug loading rate were measured. The temperature and ultrasound signal change were evaluated after 808nm Near-infrared laser (1W/cm2, 5min) irradiation. Moreover, the biosafety of FeTA/PLGA/PFP nanoparticles was assessed in vitro. The following experimental groups were established according to different types of nanoparticles: Control group; Laser group; FeTA/PLGA/PTX/PFP (FPTP)group; FeTA/PLGA/PTX/PFP +Laser (FPTP+Laser) group; Different concentration of nanoparticles (0、0.125、0.25、0.5、1.0mg/ml) in FPTP+Laser group. Furthermore, the killing effects on Y79 cells among all aforementioned groups were evaluated after irradiation for 5 minutes. Results FeTA/PLGA/PTX/PFP nanoparticles were prepared successfully. The average particle size of nanoparticles was (155.8±55.68) nm and the mean surface charge was (-27.3±5.14) mV. Gray core with black FeTA coated nanoparticles could be seen under transmission electron microscope.The encapsulation efficiency and drug loading rate of particles were (70.89±8.03) % and (9.61±0.63) %, respectively. In addition, temperature increased when applying irradiation in vitro and was positively correlated with the concentration of nanoparticles. Moreover, the ultrasound signals in B-mode and Imaging-mode enhanced with increasing concentration of the nanoparticles. It was shown that the killing effect against Y79 in FPTP+laser group was stronger than that of controll group, laser group and FTTP group in vitro, which is positively correlated with the concentration of nanoparticles. Conclusions The phase changeable nanoparticles loaded with Fe-tannic acid and paclitaxelon were successfully prepared. Such particles possess excellent photothermal effect and ultrasound imaging effect along with a robust killing effect on Y79 cells, which is expected to realize the integratedSapproach toSdiagnosisSandStreatment for RB.
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