| 康正樾,冯潇铃,廖洪建,张志飞,杜永洪.制备纳米粒联合高强度聚焦超声治疗三阴性乳腺癌：体外实验[J].中国医学影像技术,2022,38(7):961~967 |
| 制备纳米粒联合高强度聚焦超声治疗三阴性乳腺癌：体外实验 |
| Preparation of nanoparticle combining with high intensity focused ultrasound for treating three negative breast cancer：In vitro experiment |
| 投稿时间：2021-11-03 修订日期：2022-03-06 |
| DOI：10.13929/j.issn.1003-3289.2022.07.001 |
| 中文关键词: 三阴性乳腺肿瘤 纳米微粒 液态氟碳 高强度聚焦超声消融术 超声检查 |
| 英文关键词:triple negative breast neoplasms nanoparticles perfluorooctylbromide high-intensity focused ultrasound ablation ultrasonography |
| 基金项目:重庆市技术创新与应用发展专项(cstc2019jscx-msxmX0255)。 |
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| 中文摘要: |
| 目的 制备特异性靶向三阴性乳腺癌(TNBC)纳米粒(NP)AS1411/PFH-NP,评估其联合高强度聚焦超声(HIFU)治疗TNBC的价值。方法 以双乳化法将十四氟己烷(PFH)装载于PLGA@PEG,以AS1411核酸适配体与PFH-NP连接,制备AS1411/PFH-NP,观察其物理特性、靶向能力及体外超声显像能力,评价HIFU联合AS1411/PFH-NP消融离体牛肝组织及杀伤MB-231 TNBC细胞的价值。结果 成功制备AS1411/PFH-NP,其形态较佳,对MB-231 TNBC细胞具有良好靶向能力。AS1411/PFH-NP组CEUS图像灰度值大于磷酸盐缓冲液(PBS)组及NP组(t=17.24、5.60,P均＜0.05)。经HIFU+AS1411/PFH-NP消融后,牛肝组织凝固性坏死体积大于经HIFU+PBS(t=7.99,P＜0.01)及HIFU+NP消融后(t=4.33,P＜0.05);且HIFU+AS1411/PFH-NP消融能效因子显著低于HIFU+PBS(t=5.41,P＜0.01)及HIFU+NP消融(t=4.82,P＜0.01)。HIFU+AS1411/PFH-NP组MB-231细胞凋亡率高于其他组(P均＜0.05)。结论 特异性靶向TNBC AS1411/PFH-NP可提高HIFU体外消融效率。 |
| 英文摘要: |
| Objective To prepare nanoparticle (NP) AS1411/PFH-NP that specifically targeting triple negative breast cancer (TNBC), and to observe the value of AS1411/PFH-NP combining with high intensity focused ultrasound (HIFU) for treating TNBC. Methods The liquid fluorocarbon (PFH) was loaded into PLGA@PEG using double emulsification method, and the aptamer AS1411 was connected with PFH-NP to prepare AS1411/PFH-NP. The physical properties, targeting ability and ultrasonic imaging ability of AS1411/PFH-NP were observed. The value of HIFU combining with AS1411/PFH-NP for ablation of isolated bovine liver and killing MB-231 TNBC cells were analyzed. Results AS1411/ PFH-NP was successfully prepared, which had good morphology and good targeting ability to MB-231 TNBC cells. The gray value of CEUS of AS1411/PFH-NP group was higher than that of phosphate buffer solution (PBS) group and NP group (t=17.24, 5.60, both P＜0.05). The volume of coagulation necrosis in bovine liver tissue after HIFU+ AS1411/PFH-NP ablation was larger than that after HIFU+PBS (t=7.99, P＜0.01) and HIFU+NP (t=4.33, P＜0.05) ablations, and the energy efficiency factor of HIFU+AS1411/PFH-NP ablation was significantly lower than that of HIFU+PBS (t=5.41, P＜0.01) and HIFU+NP (t=4.82, P＜0.01) ablations. The apoptosis rate of MB-231 cells of HIFU+ AS1411/PFH-NP group was higher than that of the other groups (all P＜0.05). Conclusion AS1411/PFH-NP that specifically targeting TNBC could improve the ablation efficiency of HIFU in vitro. |
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