康正樾,冯潇铃,廖洪建,张志飞,杜永洪.制备纳米粒联合高强度聚焦超声治疗三阴性乳腺癌:体外实验[J].中国医学影像技术,2022,38(7):961~967
制备纳米粒联合高强度聚焦超声治疗三阴性乳腺癌:体外实验
Preparation of nanoparticle combining with high intensity focused ultrasound for treating three negative breast cancer:In vitro experiment
投稿时间:2021-11-03  修订日期:2022-03-06
DOI:10.13929/j.issn.1003-3289.2022.07.001
中文关键词:  三阴性乳腺肿瘤  纳米微粒  液态氟碳  高强度聚焦超声消融术  超声检查
英文关键词:triple negative breast neoplasms  nanoparticles  perfluorooctylbromide  high-intensity focused ultrasound ablation  ultrasonography
基金项目:重庆市技术创新与应用发展专项(cstc2019jscx-msxmX0255)。
作者单位E-mail
康正樾 重庆医科大学生物医学工程学院 超声医学工程国家重点实验室 重庆市生物医学工程学重点实验室, 重庆 400016  
冯潇铃 重庆医科大学生物医学工程学院 超声医学工程国家重点实验室 重庆市生物医学工程学重点实验室, 重庆 400016  
廖洪建 重庆医科大学生物医学工程学院 超声医学工程国家重点实验室 重庆市生物医学工程学重点实验室, 重庆 400016  
张志飞 重庆医科大学生物医学工程学院 超声医学工程国家重点实验室 重庆市生物医学工程学重点实验室, 重庆 400016  
杜永洪 重庆医科大学生物医学工程学院 超声医学工程国家重点实验室 重庆市生物医学工程学重点实验室, 重庆 400016 duyonghong@cqmu.edu.cn 
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中文摘要:
       目的 制备特异性靶向三阴性乳腺癌(TNBC)纳米粒(NP)AS1411/PFH-NP,评估其联合高强度聚焦超声(HIFU)治疗TNBC的价值。方法 以双乳化法将十四氟己烷(PFH)装载于PLGA@PEG,以AS1411核酸适配体与PFH-NP连接,制备AS1411/PFH-NP,观察其物理特性、靶向能力及体外超声显像能力,评价HIFU联合AS1411/PFH-NP消融离体牛肝组织及杀伤MB-231 TNBC细胞的价值。结果 成功制备AS1411/PFH-NP,其形态较佳,对MB-231 TNBC细胞具有良好靶向能力。AS1411/PFH-NP组CEUS图像灰度值大于磷酸盐缓冲液(PBS)组及NP组(t=17.24、5.60,P均<0.05)。经HIFU+AS1411/PFH-NP消融后,牛肝组织凝固性坏死体积大于经HIFU+PBS(t=7.99,P<0.01)及HIFU+NP消融后(t=4.33,P<0.05);且HIFU+AS1411/PFH-NP消融能效因子显著低于HIFU+PBS(t=5.41,P<0.01)及HIFU+NP消融(t=4.82,P<0.01)。HIFU+AS1411/PFH-NP组MB-231细胞凋亡率高于其他组(P均<0.05)。结论 特异性靶向TNBC AS1411/PFH-NP可提高HIFU体外消融效率。
英文摘要:
      Objective To prepare nanoparticle (NP) AS1411/PFH-NP that specifically targeting triple negative breast cancer (TNBC), and to observe the value of AS1411/PFH-NP combining with high intensity focused ultrasound (HIFU) for treating TNBC. Methods The liquid fluorocarbon (PFH) was loaded into PLGA@PEG using double emulsification method, and the aptamer AS1411 was connected with PFH-NP to prepare AS1411/PFH-NP. The physical properties, targeting ability and ultrasonic imaging ability of AS1411/PFH-NP were observed. The value of HIFU combining with AS1411/PFH-NP for ablation of isolated bovine liver and killing MB-231 TNBC cells were analyzed. Results AS1411/ PFH-NP was successfully prepared, which had good morphology and good targeting ability to MB-231 TNBC cells. The gray value of CEUS of AS1411/PFH-NP group was higher than that of phosphate buffer solution (PBS) group and NP group (t=17.24, 5.60, both P<0.05). The volume of coagulation necrosis in bovine liver tissue after HIFU+ AS1411/PFH-NP ablation was larger than that after HIFU+PBS (t=7.99, P<0.01) and HIFU+NP (t=4.33, P<0.05) ablations, and the energy efficiency factor of HIFU+AS1411/PFH-NP ablation was significantly lower than that of HIFU+PBS (t=5.41, P<0.01) and HIFU+NP (t=4.82, P<0.01) ablations. The apoptosis rate of MB-231 cells of HIFU+ AS1411/PFH-NP group was higher than that of the other groups (all P<0.05). Conclusion AS1411/PFH-NP that specifically targeting TNBC could improve the ablation efficiency of HIFU in vitro.
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