| 黎成,卜超,苏赟,钟嘉宝,潘爱珍,高明勇,黄穗乔.制备MRI双靶向分子示踪剂细胞黏附分子-微米级氧化铁颗粒及体外实验[J].中国医学影像技术,2019,35(6):805~811 |
| 制备MRI双靶向分子示踪剂细胞黏附分子-微米级氧化铁颗粒及体外实验 |
| Preparation of MRI contrast of dual-targeted cell adhesion molecules with microparticles of iron oxide and in vitro experiment |
| 投稿时间：2018-11-14 修订日期：2019-04-02 |
| DOI：10.13929/j.1003-3289.201811071 |
| 中文关键词: 脑损伤 细胞黏附分子-1 微米级氧化铁颗粒 分子磁共振成像 |
| 英文关键词:brain injuries cell adhesion molecule-1 microparticles of iron oxide molecular magnetic resonance imaging |
| 基金项目:广东省公益研究与能力建设专项基金（2014A020212054）、佛山市医学重点专科培育项目建设（Fspy3-2015013）。 |
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| 中文摘要: |
| 目的 观察新型MRI双靶向分子示踪剂细胞黏附分子-微米级氧化铁颗粒（CAM-MPIO）与体外内皮细胞的结合能力。方法 制备单靶向分子示踪剂细胞间黏附分子（ICAM）-MPIO、血管细胞黏附分子（VCAM）-MPIO和双靶向分子示踪剂CAM-MPIO，将其与肿瘤坏死因子-α炎性激活的内皮细胞结合，采用普鲁士蓝染色法、免疫荧光法及MR检测其特异性结合能力。结果 普鲁士蓝染色结果显示CAM-MPIO组的蓝染铁颗粒分布较ICAM-MPIO组、VCAM-MPIO组明显增多。CAM-MPIO组细胞周围黄色荧光面积分别为ICAM-MPIO、VCAM-MPIO组的（2.00±0.31）倍和（2.46±0.45）倍。T2WI信号强度和T2值随靶向分子示踪剂浓度增高呈不同程度减低，且以CAM-MPIO组减低为著。结论 制备的双靶向分子示踪剂与内皮细胞结合能力优于单靶向分子示踪剂，有望用于早期影像学诊断放射性脑损伤。 |
| 英文摘要: |
| Objective To evaluate the specificity and efficacy of combination of dual-targeted MRI contrast agent cell adhesion molecule (CAM)-microparticles of iron oxide (MPIO) and endothelial cells. Methods The single-targeted contrast agent intercellular adhesion molecule (ICAM)-MPIO, vascular cell adhesion molecule (VCAM)-MPIO and dual-targeted contrast agent CAM-MPIO were synthesized, then Prussian blue staining, immunofluorescence and MR scanning were applied to estimate the specificity and efficacy of combination of contrast agents and tumor necrosis factor-α (TNF-α) activated endothelial cells. Results Prussian blue staining showed much stronger blue granules surrounding the stimulated cells in CAM-MPIO group than in ICAM-MPIO group and VCAM-MPIO group. Immunofluorescence essay demonstrated that the yellow fluorescence area per cell of CAM-MPIO group was (2.00±0.31) times and (2.46±0.45) times higher than that of ICAM-MPIO group and VCAM-MPIO group. In vitro MRI showed the signal intensity of T2WI and T2 value decreased with the increase concentration of targeted contrast agents, especially in CAM-MPIO group. Conclusion Dual-targeted probe CAM-MPIO may be more valuable than single-targeted probe ICAM-MPIO and VCAM-MPIO for imaging diagnosis of early radiation-induced brain injury. |
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