| 徐曼,余永强,侯唯姝,潘红利.体素内不相干运动DWI评估SD大鼠C6脑胶质瘤模型肿瘤微环境乏氧状态[J].中国医学影像技术,2019,35(2):170~174 |
| 体素内不相干运动DWI评估SD大鼠C6脑胶质瘤模型肿瘤微环境乏氧状态 |
| Intravoxel incoherent motion DWI in assessment of hypoxic status of tumor microenvironment of C6 glioma modle in SD rats |
| 投稿时间：2018-06-13 修订日期：2018-11-04 |
| DOI：10.13929/j.1003-3289.201806071 |
| 中文关键词: 神经胶质瘤 磁共振成像 体素内不相干运动 缺氧诱导因子1 |
| 英文关键词:glioma magnetic resonance imaging intravoxel incoherent motion imaging hypoxia-inducible factor 1 |
| 基金项目:合肥市借转补项目（YW201608080003）。 |
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| 中文摘要: |
| 目的 观察体素内不相干运动DWI（IVIM-DWI）评估SD大鼠C6脑胶质瘤模型肿瘤微环境乏氧状态的可行性。方法 建立SD大鼠C6脑胶质瘤模型（n=48），于造模第14、21及28天分别取16只大鼠行IVIM-DWI，获得ADC、纯扩散系数（D）、伪灌注扩散系数（D*）及灌注分数（f）。每次扫描后处死大鼠，取肿瘤组织行缺氧诱导因子1α（HIF-1α）免疫组织化学化染色并评分，比较造模后各时间点IVIM-DWI参数及HIF-1α评分差异，分析IVIM-DWI参数与HIF-1α评分的相关性。结果 造模第14、21及28天，IVIM-DWI各参数及HIF-1α评分比较差异均有统计学意义（P均<0.01）。造模第21天，D、D*、f均与HIF-1α评分呈负相关（r=-0.73、-0.58、-0.67，P均<0.05）；造模第28天，D、f均与HIF-1α评分呈负相关（r=-0.60、-0.65，P均<0.05）。结论 IVIM-DWI能够评价SD大鼠C6脑胶质瘤模型肿瘤微环境乏氧状态。 |
| 英文摘要: |
| Objective To explore the feasibility of intravoxel incoherent motion DWI (IVIM-DWI) in assessment of hypoxic status of tumor microenvironment of C6 glioma in SD rat models. Methods C6 glioma models were established in 48 SD rats. IVIM-DWI scanning were performed on 16 rats on the 14th day, 21st day and 28th day after modeling, respectively, and parameters of ADC, slow diffusion coefficient (D), fast diffusion coefficient (D*) and fractional perfusion-related volume (f) were obtained. The rats were scarified after each scanning, and the tumors were removed for hypoxia-inducible factor (HIF-1α) immunohistochemical staining and scoring. Differences of IVIM-DWI parameters and HIF-1α score at different time points were compared, and correlation between parameters of IVIM-DWI and HIF-1α score were analyzed. Results There were statistical differences of IVIM-DWI parameters and HIF-1α score on the 14th day, 21st day and 28th day after modeling (all P<0.01). On the 21st day after modeling, HIF-1α score was negatively correlated with D, D* and f (r=-0.73, -0.58, -0.67, all P<0.05), while on the 28th day after modeling, HIF-1α score were negatively correlated with D and f (r=-0.60, -0.65, both P<0.05). Conclusion IVIM-DWI can be used to assess hypoxic status of tumor microenvironment of C6 glioma SD rat models. |
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