| 李卉,王德玲,尹韶晗,耿志君,谢传淼.ADC值反映EB病毒LMP1基因表达的可行性[J].中国医学影像技术,2017,33(2):167~170 |
| ADC值反映EB病毒LMP1基因表达的可行性 |
| Feasibility of apparent diffusion coefficient values in reflecting expression of Epstein-Barr virus-latent membrane protein 1 gene |
| 投稿时间：2016-07-28 修订日期：2016-11-24 |
| DOI：10.13929/j.1003-3289.201607065 |
| 中文关键词: 模型,动物 潜伏膜蛋白基因1 扩散磁共振成像 表观扩散系数 |
| 英文关键词:Models,animal Latent membrane protein 1 gene Diffusion magnetic resonance imaging Apparent diffusion coefficient |
| 基金项目:广东省医学科研基金（A2015377）。 |
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| 中文摘要: |
| 目的 探讨ADC值反映EB病毒潜伏膜蛋白质1（LMP1）基因表达的可行性。方法 建立LMP1（+）组 和LMP1（-）组 的皮下种植瘤裸鼠模型（每组15只）。待移植瘤体积达90~110 mm3后，对实验动物进行常规MR及DWI检查，分别获得两组肿瘤的ADC值，并进行统计学分析。实验结束后，观察两组实验裸鼠种植瘤病理组织学改变。结果 LMP1（+）组肿瘤体积 明显大于LMP1（-）组。常规MR扫描示LMP1（+）组肿瘤内坏死较LMP1（-）组更明显。LMP1（+）组肿瘤ADC值为（0.68±0.12）×10-3mm/s2，LMP1（-）组肿瘤ADC值为（0.87±0.23）×10-3mm/s2，差异有统计学意义（t=9.34，P<0.01）。肿瘤病理组织学检查显示与LMP1（-）组相比，LMP1（+）组肿瘤细胞更密集，坏死更多见。结论 ADC值可有效反映裸鼠移植瘤中EB病毒LMP1基因的表达。 |
| 英文摘要: |
| Objective To evaluate the feasibility of ADC in reflecting the expression of Epstein-Barr virus (EBV)-latent membrane protein 1 gene (EBV-LMP1).Methods A total 30 nude mice model were divided into two groups, namely LMP1(+) group (included CNE2-LMP1 subgroup, HONE1-LMP1 subgroup) and LMP1 (-) group (included CNE2-LMP1 subgroup and HONE1-LMP1 subgroup; each n=15). The MRI and DWI were performed when the tumor volumes were 90-110 mm3. The ADC values of each group were obtained and statistical analysis. Histological specimens of each group were obtained after the completion of image acquisition.Results The tumors volume of LMP1 (+) group mm3) were significant larger than that of LMP1 (-) group mm3;t=6.31, P=0.03). LMP1 (+) group had more necrosis in the lesions than LMP1 (-) group in MRI scan. The ADC values of LMP1 (+) (×10-3mm/s2) were significant lower than that of LMP1 (-) group (×10-3mm/s2; t=9.34, P<0.01). Histopathological examination showed the cells of the LMP (+) group were more dense and necrosis were more easily to be detected, compared to LMP1 (-) group.Conclusion ADC value can effectively reflect the expression of EVB-LMP1. |
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